Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody | Alomone Labs ASR-036-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody

catalog :

ASR-036-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of 5HT4 receptor in live intact mouse J774 macrophage cells: Cell surface detection of 5HT4 receptor in live intact mouse J774 macrophage cells: Black line: Cells. Black line: Cells + rabbit IgG isotype control-FITC. Black line: Cells + Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (#ASR-036-F), (2.5 µg).

product information

CAT :

ASR-036-F

SKU :

ASR-036-F_50 mcl

Product Name :

Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62758

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR036

Homology :

Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Product Page - Scientific background :

5-hydroxytryptamine receptor 4 (5-HT4 receptor) is a subclass among the large family of receptors mediating serotonin signaling at central and peripheral targets. 5-HT4 receptor activation plays a role in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.5-HT4 receptor is a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs) and couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs); the N-terminus is on the extracellular side of the membrane, and the C-terminus is cytoplasmic3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (#ASR-036) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications. It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples. Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (#ASR-036-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASR-036), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage