Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs ASR-035-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody

catalog :

ASR-035-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of 5-Hydroxytryptamine receptor 2B in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-035-AG), (1:80). A. 5-HT2B staining (green) appears in neurons of the Purkinje layer (vertical arrows) and in the molecular layer (horizontal arrow). B. Nuclear staining using DAPI as the counterstaining (blue). C. Merged image of panels A and B.

image 2 :

Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 5) human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 6) human K562 chronic myeloid leukemia cell line lysate (lanes 3 and 7) and human Jurkat T-cell leukemia cell line lysate (lanes 4 and 8): - 1-4. Anti-GLUT1 (extracellular) Antibody (#AGT-041) (1:400).5-8. Anti-GLUT1 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ASR-035-AG

SKU :

ASR-035-AG_50 mcl

Product Name :

Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30994

Applications :

FC IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR035

Homology :

Mouse - identical, human - 11/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KMSEQ STISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B

Product Page - Scientific background :

5-HT is involved in a variety of physiological and biological processes. In the brain, 5-HT has been found to affect sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. Like other members, they are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2B receptors have been found to be present in both rodent and human tissues, particularly in the cardiovascular system, gastrointestinal tract, bone, and central nervous system2. Only low expression levels are found in the brain and blood. The highest expression levels are found in the stomach fundus3.Over-expression of 5-HT2B receptors in hearts of transgenic mice results in cardiac hypertrophy and decreased ventricular function due to enhanced extracellular matrix (ECM) and remodeling. Likewise, genetic deletion of 5-HT2B receptors was shown to lead to ventricular dilation and incomplete cardiac development5.

Applications may also work in :

FC IHC LCI

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035) is a highly specific antibody directed against an epitope of the rat 5-HT-2B receptor. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5HT2B receptor in living cells. It has been designed to recognize 5-hydroxytryptamine receptor 2B from rat, mouse and human samples. Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-035-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR2B

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASR-035), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:80

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage