antibody

Anti-5HT2C Receptor (HTR2C) Antibody

ASR-034

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

ASR-034-CF_0.2 ml

Anti-5HT2C Receptor (HTR2C) Antibody

Antibodies

Antibodies

Polyclonal

P08909

IHC WB

Human Rat Mouse

Rabbit

BLP-SR034

Mouse- identical; human - 12 out of 14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)RRQTLMLLRGHTEE, corresponding to amino acid residues 243 -256 of rat HTR2C

5-hydroxytryptamine receptor 2C, 5HT2C/5HT2CR, also known as serotonin receptor 2C, is a transmembrane (TM) receptor subtype in the 5-HT2R family of class A G-protein coupled receptors (GPCRs). 5HT2CR is encoded by the HTR2C gene and mediates various physiological functions, including disease-related pathways and behaviors due to its effects on the central nervous system (CNS).1,2The 5HT2C receptor is composed of seven TM helices (TM I - VII), three extracellular (ECL 1-3) and three intracellular loops (ICL 1-3), an intracellular carboxy-terminus, and an extracellular amino-terminus. Activation of 5HT2CR through 5-HT binding to Gαq/11 triggers G protein-dependent signaling that activates phospholipase Cβ (PLCβ) and leads to phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis to produce inositol-1,4,5-trisphosphate (IP3), accumulation of the downstream IP3 metabolite inositol monophosphate (IP1), and diacylglycerol (DAG), resulting in increased levels of intracellular mobilization of Ca2+ and IP1.1Activation of 5HT2CR can regulate ion channels and transport processes, as well as activate other downstream effectors including phospholipase A2 (PLA2), phospholipase D (PLD), cyclic nucleotides, and extracellular signal-regulated kinases (ERK1/2). Stimulation of 5HT2CR can activate cytosolic PLA2 to produce free arachidonic acid and its metabolites. Depending on the ligand, agonist signaling bias can activate either PLCβ or PLA2. Activation of 5HT2CR leads to protein kinase C (PKC) activation and downstream stimulation of the mitogen-activated protein kinase cascade, resulting in ERK1/2 phosphorylation. 5HT2CR has been shown to couple ERK1/2 through a PLD- and PKC-dependent pathway, potentially with Gα12/13 proteins.15HT2CR has been shown to interact with proteins including PSD-95/disk large/zonula occludens domain-containing proteins, calmodulin, β-arrestins, and phosphatase and tensin homolog, which all modulate receptor kinetics. β-arrestins play a significant role in regulating the functional activity of 5HT2CR by controlling desensitization and resensitization processes. Agonist-dependent desensitization of 5HT2CR involves phosphorylation by G-protein receptor kinase 2 (GRK2), binding of β-arrestin, and uncoupling of the receptor from G protein resulting in subsequent internalization into endosomes. Resensitization and recycling back to the plasma membrane occur upon dephosphorylation.1The expression and function of 5HT2CR play a primary role in the CNS. Serotonergic cell bodies in the midbrain dorsal raphe nuclei project to various brain regions, including the ventral tegmental area (VTA), nucleus accumbens (NAc), and prefrontal cortex (PFC), which are associated with reward and executive function. 5HT2CR is expressed postsynaptically in various neuronal cell types that use neurotransmitters such as acetylcholine, dopamine, and γ-aminobutyric acid (GABA), with a significant outcome of 5HT2CR stimulation being the modulation of dopamine neuronal function.15HT2CR plays a crucial role in regulating several physiological brain functions such as food intake, anxiety, stress response, motoneuron activity, and sleep. Dysregulation of 5HT2CR is observed in conditions such as depression, schizophrenia, suicidal behavior, spinal cord injury, and obesity.2

IHC WB

Alomone Labs

5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C

A Rabbit Polyclonal antibody to 5HT2C Receptor (HTR2C)

Anti-5HT2C Receptor (HTR2C) Antibody (#ASR-034) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5HT2C from rat, mouse and human samples.

BLP-SR034

NA

5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

HTR2C

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:200