Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody | Alomone Labs ASR-033 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody

catalog :

ASR-033

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Wen X, Yu X, Huo R, Yan Q, Wu D, Feng Y, et al. Serotonin-Mediated Cardiac Analgesia via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction. Neuroscience. 2019;411:150-163 pubmed publisher
Olianas M, Dedoni S, Onali P. LPA1 Mediates Antidepressant-Induced ERK1/2 Signaling and Protection from Oxidative Stress in Glial Cells. J Pharmacol Exp Ther. 2016;359:340-353 pubmed
Chang W, Kanda H, Ikeda R, Ling J, DeBerry J, Gu J. Merkel disc is a serotonergic synapse in the epidermis for transmitting tactile signals in mammals. Proc Natl Acad Sci U S A. 2016;113:E5491-500 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG (lanes 1 and 4), mouse brain (lanes 2 and 5) and human brain neuroblastoma SH-SY5Y (lanes 3 and 6) lysates: - 1-3. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:200).4-6. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody, preincubated with 5HT2A Receptor/HTR2A (extracellular) Blocking Peptide (#BLP-SR033).

image 2 :

Expression of 5HT2A Receptor in rat cingulate cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. 5HT2A Receptor immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the antibody with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (BLP-SR033), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Immuno-colocalization of VGLUT2 and Neurokinin 1 Receptor in rat DRG - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sectionsusingAnti-VGLUT2-ATTO-594 Antibody(#AGC-036-AR) (1:60) andAnti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO-488 Antibody(#ATR-001-AG) (1:60). A. VGLUT2 staining (red). B. NK1 receptor staining (green). C. Merge of the two images demonstrates co-localization in some neuronal bodies (arrows point at examples). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ASR-033

SKU :

ASR-033-CF_0.2 ml

Product Name :

Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P14842

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR033

Homology :

Mouse - identical; human - 9/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A

Product Page - Scientific background :

5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit-hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat 5-HT-2A receptor. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033) can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is ideal for detecting the receptor in living cells. It has been designed to recognize rat 5-hydroxytryptamine receptor 2A from rat, mouse, and human samples.

Negative Control :

BLP-SR033

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR2A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage