Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody | Alomone Labs ASR-033-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody

catalog :

ASR-033-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1, 2. Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP), (1:200). 3, 4. Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody, preincubated with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (#BLP-SR033).

image 2 :

Expression of 5-HT2A in rat dorsal raphe nucleus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP), (1:400), followed by goat anti-guinea pig - Alexa Fluor-594. A. 5-HT2A immunoreactivity (red) appears in neuronal outlines (arrows). B. Pre-incubation of the antibody with 5HT2A Receptor (HTR2A) (extracellular) Blocking Peptide (#BLP-SR033), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ASR-033-GP

SKU :

ASR-033-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P14842

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-SR033

Homology :

Mouse - identical; human - 9 out of 13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A

Product Page - Scientific background :

5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit-hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A, 5-HT2A

Short Description :

A Guinea Pig Polyclonal Antibody to 5-HT2A Receptor

Long Description :

Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (#ASR-033-GP) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5-HT2A from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-5HT2A Receptor (HTR2A) (extracellular) (#ASR-033) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-SR033

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A, 5-HT2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR2A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage