Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody | Alomone Labs ASR-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody

catalog :

ASR-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wen X, Yu X, Huo R, Yan Q, Wu D, Feng Y, et al. Serotonin-Mediated Cardiac Analgesia via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction. Neuroscience. 2019;411:150-163 pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus lysate (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4): - 1,2. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), (1:200).3,4. Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody, preincubated with 5HT3B Receptor/HTR3B (extracellular) Blocking Peptide (#BLP-SR032).

image 2 :

Expression of Serotonin receptor 3B in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. 5-HT3B staining (green) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counter stain. C. Merged image of A and B.

image 3 :

Expression of Serotonin receptor 3B in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody(#ASR-032) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. A. 5-HT3B staining (green) appears in neuronal cell bodies. B. Nuclear staining using DAPI as the counter stain. C. Merged image of A and B.

product information

CAT :

ASR-032

SKU :

ASR-032-CF_0.2 ml

Product Name :

Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JJ16

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR032

Homology :

Mouse - identicalHuman- not recommended for use with human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B

Product Page - Scientific background :

5-Hydroxytryptamine receptor 3B (5-HT3B, HTR3B) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The localization of 5-HT3B subunits is somewhat controversial; some studies show that its expression is restricted to the peripheral nervous system and others show that it is also detected in the brain and in hippocampal neurons1,4-7.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B

Long Description :

Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3B. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5-HT3B in living cells. It has been designed to recognize 5-HT3B from rat and mouse samples. The antibody is unlikely to recognize the receptor from human samples.

Negative Control :

BLP-SR032

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR3B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage