antibody

Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody

ASR-032-AG

polyclonal

domestic rabbit

ATTO 488

NA

mouse, rat

immunohistochemistry, immunocytochemistry, flow cytometry

ASR-032-AG_50 mcl

Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody

Antibodies

Antibodies

Polyclonal

Q9JJ16

FC IF IHC

Rat Mouse

Rabbit

BLP-SR032

Mouse - identicalHuman- not recommended for use with human samples

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B

5-Hydroxytryptamine receptor 3B (5-HT3B, HTR3B) belongs to the super-family of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the super-family of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The localization of 5-HT3B subunits is somewhat controversial; some studies show that its expression is restricted to the peripheral nervous system and others show that it is also detected in the brain and in hippocampal neurons1,4-7.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

FC IF IHC

Alomone Labs

5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B Conjugated to the Fluorescent Dye ATTO-488

Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (#ASR-032) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3B. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5-HT3B in living cells. It has been designed to recognize 5-HT3B from rat and mouse samples. The antibody is unlikely to recognize the receptor from human samples. Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-032-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

RIC-001-AG

NA

5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

HTR3B

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ASR-032), and immunohistochemistry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information