Anti-5HT3A Receptor (HTR3A) Antibody | Alomone Labs ASR-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT3A Receptor (HTR3A) Antibody

catalog :

ASR-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Patil M, Hovhannisyan A, Wangzhou A, Mecklenburg J, Koek W, Goffin V, et al. Prolactin receptor expression in mouse dorsal root ganglia neuronal subtypes is sex-dependent. J Neuroendocrinol. 2019;31:e12759 pubmed publisher
Wen X, Yu X, Huo R, Yan Q, Wu D, Feng Y, et al. Serotonin-Mediated Cardiac Analgesia via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic Infarction. Neuroscience. 2019;411:150-163 pubmed publisher
Salaga M, Binienda A, Piscitelli F, Mokrowiecka A, Cygankiewicz A, Verde R, et al. Systemic administration of serotonin exacerbates abdominal pain and colitis via interaction with the endocannabinoid system. Biochem Pharmacol. 2019;161:37-51 pubmed publisher
Lombaert N, Hennes M, Gilissen S, Schevenels G, Aerts L, Vanlaer R, et al. 5-HTR2A and 5-HTR3A but not 5-HTR1A antagonism impairs the cross-modal reactivation of deprived visual cortex in adulthood. Mol Brain. 2018;11:65 pubmed publisher
Patil M, Hovhannisyan A, Akopian A. Characteristics of sensory neuronal groups in CGRP-cre-ER reporter mice: Comparison to Nav1.8-cre, TRPV1-cre and TRPV1-GFP mouse lines. PLoS ONE. 2018;13:e0198601 pubmed publisher
Thompson A, Metzger S, Lochner M, Ruepp M. The binding orientation of epibatidine at ?7 nACh receptors. Neuropharmacology. 2017;116:421-428 pubmed publisher
Chang W, Kanda H, Ikeda R, Ling J, DeBerry J, Gu J. Merkel disc is a serotonergic synapse in the epidermis for transmitting tactile signals in mammals. Proc Natl Acad Sci U S A. 2016;113:E5491-500 pubmed publisher
Chen H, Hong F, Chen Y, Li J, Yao Y, Zhang Y, et al. Activation of islet 5-HT4 receptor regulates glycemic control through promoting insulin secretion. Eur J Pharmacol. 2016;789:354-361 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:200).3,4. Anti-5HT3A Receptor (HTR3A) Antibody, preincubated with 5HT3A Receptor/HTR3A Blocking Peptide (#BLP-SR031).

image 2 :

Expression of Serotonin receptor 3A in rat brain - Immunohistochemical staining of rat parietal cortex using Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031), (1:400), (red). A. 5-HT3A staining appears in neuronal outlines (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

image 3 :

Expression of Serotonin receptor 3Ain rat brain - Immunohistochemical staining of rat parietal cortex using Anti-5HT3A Receptor (HTR3A) Antibody(#ASR-031) (1:400) (red). A. 5-HT3A staining appears in neuronal outlines (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

product information

CAT :

ASR-031

SKU :

ASR-031-CF_0.2 ml

Product Name :

Anti-5HT3A Receptor (HTR3A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35563

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR031

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine receptor 3A

Product Page - Scientific background :

5-Hydroxytryptamine receptor 3A (5-HT3A, HTR3A) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The 5-HT3A subunit is expressed in GABAergic and enkephalinergic neurons in the spinal dorsal horn thereby marking its possible antinociceptive effect4.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor 3A

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3A

Long Description :

Anti-5HT3A Receptor (HTR3A) Antibody (#ASR-031) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3A. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5-HT3A from mouse, human, and rat samples.

Negative Control :

BLP-SR031

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor 3A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR3A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage