Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody | Alomone Labs ASR-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody

catalog :

ASR-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat prostate (lanes 1 and 3) and human PC-3 prostate carcinoma cell (lanes 2 and 4) lysates: - 1,2. Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody (#ASR-023), (1:200).3,4. Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody, preincubated with 5HT1D Receptor/HTR1D (extracellular) Blocking Peptide (#BLP-SR023).

image 2 :

Peptide (C)EASNRSLNATGAWD corresponding to amino acid residues 14-27 of rat 5-hydroxytryptamine receptor 1D (AccessionP28565). Extracellular N-terminus.

product information

CAT :

ASR-023

SKU :

ASR-023-CF_0.2 ml

Product Name :

Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28565

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR023

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EASNRSLNATGAWD, corresponding to amino acid residues 14-27 of rat 5-hydroxytryptamine receptor 1D

Product Page - Scientific background :

Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2.To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-protein coupled receptor superfamily and therefore span the membrane seven times3.Members of the 5-HT1 receptor class all couple Gi/0 thereby inhibiting adenylate cyclase.In the central nervous system, 5-HT1D is expressed in the basal ganglia, hippocampus and cortex in the rat and in the basal ganglia, midbrain and spinal cord in the human. In serotonin neurons, 5-HT1D receptors are autoreceptors thereby inhibiting the release of 5-HT. In GABA, ACh, and glutamate neurons they are heteroreceptors where they control the release of these neurotransmitters4,5.The serotonin system plays an undeniable role in the perception and processing of pain. Indeed, many migraine sufferers are prescribed triptan-based drugs which activate 5-HT1B and 5-HT1D receptors2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 1D, Serotonin receptor 1D, 5HT1D-alpha, HTR1DA

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1D

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat 5-Hydroxytryptamine receptor 1D. Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody (#ASR-023) can be used in western blot analysis. It has been designed to recognize 5HT1D receptor from human, rat and mouse samples.

Negative Control :

BLP-SR023

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 1D, Serotonin receptor 1D, 5HT1D-alpha, HTR1DA

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR1D

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage