Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody | Alomone Labs ASR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody

catalog :

ASR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat hippocampus lysate: - 1. Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody (#ASR-022), (1:400).2. Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody, preincubated with 5HT1B Receptor/HTR1B (extracellular) Blocking Peptide (#BLP-SR022).

image 2 :

Expression of 5-Hydroxytryptamine receptor 1B in rat and mouse brain - Immunohistochemical staining of rat and mouse brain sections using Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody (#ASR-022), (1:200). A. 5-HT1B receptor staining (green) in rat hippocampal CA1 region is detected in the pyramidal layer (P) and apical dendrites (arrows). B. 5-HT1B receptor staining (green) in mouse deep cerebellar nucleus appears in neuronal profiles (arrows). DAPI counterstain is used for nuclei staining.

product information

CAT :

ASR-022

SKU :

ASR-022-CF_0.2 ml

Product Name :

Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28222

Applications :

IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR022

Homology :

Rat - 15/17 amino acid residues identical; mouse - 14/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B

Product Page - Scientific background :

Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2.To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-protein coupled receptor superfamily and therefore span the membrane seven times3.The 5-hydroxytryptamine receptor 1B (5-HT1B) is involved in several physiological behaviors and psychiatric diseases, including locomotor activity, drug abuse reinforcement, migraine, anxiety states and aggressive behaviors1.Pharmacological studies suggest that 5-HT1B is expressed by both serotonergic and non-serotonergic neurons. In the nervous system, this receptor is located in the axon terminal of the plasma membrane of unmyelinated axons. It is predominantly localized on pre-synaptic membranes. Signal transduction studies of the receptor indicate that this receptor is coupled negatively to adenylyl cyclase as its activation induces a decrease in adenylyl cyclase activity1.

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B

Long Description :

Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody (#ASR-022) is a highly specific antibody directed against an epitope of the human 5-Hydroxytryptamine receptor 1B. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5HT1B receptor from human, rat, and mouse samples.

Negative Control :

BLP-SR022

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR1B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage