Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs ASR-022-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody

catalog :

ASR-022-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of 5-Hydroxytryptamine receptor 1B in rat and mouse brain - Immunohistochemical staining of rat and mouse brain sections using Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-022-AG), (1:80). A. 5-HT1B receptor staining (green) in rat hippocampal CA1 region is detected near the pyramidal layer (P) and in apical dendrites (arrows). B. In mouse hippocampal CA1 region, 5-HT1B receptor staining (green) appears in the pyramidal layer (P) and in apical dendrites. Nuclei are stained using DAPI (blue).

product information

CAT :

ASR-022-AG

SKU :

ASR-022-AG_50 mcl

Product Name :

Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28222

Applications :

FC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR022

Homology :

Rat - 15/17 amino acid residues identical; mouse - 14/17 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B

Product Page - Scientific background :

Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2.To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-protein coupled receptor superfamily and therefore span the membrane seven times3.The 5-hydroxytryptamine receptor 1B (5-HT1B) is involved in several physiological behaviors and psychiatric diseases, including locomotor activity, drug abuse reinforcement, migraine, anxiety states and aggressive behaviors1.Pharmacological studies suggest that 5-HT1B is expressed by both serotonergic and non-serotonergic neurons. In the nervous system, this receptor is located in the axon terminal of the plasma membrane of unmyelinated axons. It is predominantly localized on pre-synaptic membranes. Signal transduction studies of the receptor indicate that this receptor is coupled negatively to adenylyl cyclase as its activation induces a decrease in adenylyl cyclase activity1.

Applications may also work in :

FC IF IHC LCI

Supplier :

Alomone Labs

Target :

5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2

Short Description :

A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody (#ASR-022) is a highly specific antibody directed against an epitope of the human 5-Hydroxytryptamine receptor 1B. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5HT1B receptor from human, rat, and mouse samples. Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-022-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HTR1B

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASR-022), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:80

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage