antibody

Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody

ASR-013-AG

polyclonal

domestic rabbit

ATTO 488

NA

human, mouse, rat

ASR-013-AG_50 mcl

Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody

Antibodies

Antibodies

Polyclonal

Q9Z0U9

FC

Human Rat Mouse

Rabbit

BLP-SR013

Rat - identical; human - 9/12 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse S1PR3

Sphingosine 1-phosphate (S1P) is an active byproduct of sphingomyelin metabolism. All cells can synthesize this biomolecule but the majority of its synthesis comes from erythrocytes and endothelial cells. In cases of inflammation, mast cells and platelets are the main source of sphingosine 1-phosphate1. S1P cellular functions could be intracellular, where it is synthesized. In addition, upon secretion, it circulates in the blood via its binding to high-density lipoproteins and albumin. When S1P reaches its target cell it could activate 5 different high affinity receptors belonging to the G-protein coupled receptor superfamily: Sphingosine 1-phosphate receptors, termed S1PR1-51.Stimulation of S1P receptors triggers a cascade of signaling events depending on the receptor and on the G-protein it couples. S1PR1 couples to Gi2. S1PR2 can couple to Gs, Gq or G12/13 and S1PR3-5 can couple to Gior G12/133-4. The pathways activated vary from Ca2+ mobilization, activation or inhibition of adenylate cyclase phospholipase C activation and more. Through the different signaling pathways these receptors activate, S1P1 receptors are implicated in adherens junction assembly, cytoskeletal changes, cell migration, proliferation and apoptosis5.S1PR3 is specifically detected in the brain, heart, spleen, liver, lung, thymus, kidney, testis and skeletal muscle3. S1PR1 and S1PR2 are generally expressed in the CNS, cardiovascular and immune systems1. S1PR4 is specifically expressed in the lymphoid tissue and S1PR5 in natural killer cells and olygodendrocytes1.Malfunction of S1P receptor signaling is reported in various disorders, for example multiple sclerosis and may be targets for the development of therapeutic drugs1.

FC

Alomone Labs

Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3

A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye ATTO-488

Anti-S1PR3 (EDG3) (extracellular) Antibody (#ASR-013) is a highly specific antibody directed against an extracellular epitope of mouse sphingosine 1-phosphate receptor 3. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize S1PR3 from human, rat and mouse samples. Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody (#ASR-013-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

RIC-001-AG

NA

Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

S1PR3

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #ASR-013), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information