Anti-S1PR2 (EDG5) Antibody | Alomone Labs ASR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-S1PR2 (EDG5) Antibody

catalog :

ASR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates: - 1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).3,4,7,8. Anti-S1PR2 (EDG5) Antibody, preincubated with S1PR2/EDG5 Blocking Peptide (#BLP-SR012).

image 2 :

Expression of Sphingosine 1-phosphate receptor 2 in rat lung - Immunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:100). S1PR2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain.

image 3 :

Expression of Sphingosine 1-phosphate receptor 2 in rat lung - Immunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR2 (EDG5) Antibody(#ASR-012) (1:100). S1PR2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain.

product information

CAT :

ASR-012

SKU :

ASR-012-CF_0.2 ml

Product Name :

Anti-S1PR2 (EDG5) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P52592

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR012

Homology :

Rat - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2

Product Page - Scientific background :

Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2

Short Description :

A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse sphingosine 1-phosphate receptor 2. Anti-S1PR2 (EDG5) Antibody (#ASR-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize S1PR2 from mouse, rat and human samples.

Negative Control :

BLP-SR012

Positive Control :

Synonyms :

Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

S1PR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage