Anti-S1PR1 (EDG1) (extracellular) Antibody | Alomone Labs ASR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-S1PR1 (EDG1) (extracellular) Antibody

catalog :

ASR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:50; fig 2 immunocytochemistry; mouse; fig 6 immunohistochemistry; mouse; 1:50; fig 3	Joly S, Pernet V. Sphingosine 1-phosphate receptor 1 is required for retinal ganglion cell survival after optic nerve trauma. J Neurochem. 2016;138:571-86 pubmed publisher
	Anwar M, Amin M, Balaji Ragunathrao V, Matsche J, Karginov A, Minshall R, et al. Tyrosine phosphorylation of S1PR1 leads to chaperone BiP-mediated import to the endoplasmic reticulum. J Cell Biol. 2021;220: pubmed publisher
	Liu H, Luo Z, Gu J, Jiang H, Joshi S, Shoghi K, et al. In vivo Characterization of Four 18F-Labeled S1PR1 Tracers for Neuroinflammation. Mol Imaging Biol. 2020;22:1362-1369 pubmed publisher

image

image 1 :

Western blot analysis of (RAEC) rat aortic endothelial cell line lysates (lanes 1 and 3) and A-10 Rat thoracic aorta smooth muscle (lane 2 and 4) cell lysates: - 1,2. Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:200).3,4. Anti-S1PR1 (EDG1) (extracellular) Antibody, preincubated with S1PR1/EDG1 (extracellular) Blocking Peptide (#BLP-SR011).

image 2 :

Expression of Sphingosine 1-phosphate receptor 1 in rat lung - Immunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain.

product information

CAT :

ASR-011

SKU :

ASR-011-CF_0.2 ml

Product Name :

Anti-S1PR1 (EDG1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21453

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR011

Homology :

Mouse, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1

Product Page - Scientific background :

Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1

Short Description :

A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1

Long Description :

Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize S1PR1 from human, rat, and mouse samples.

Negative Control :

BLP-SR011

Positive Control :

Synonyms :

Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

S1PR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage