Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody | Alomone Labs ASR-011-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody

catalog :

ASR-011-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Liu H, Luo Z, Gu J, Jiang H, Joshi S, Shoghi K, et al. In vivo Characterization of Four 18F-Labeled S1PR1 Tracers for Neuroinflammation. Mol Imaging Biol. 2020;22:1362-1369 pubmed publisher

image

image 1 :

Cell surface detection of S1PR1 in live intact human Jurkat T-cell leukemia cells: Cell surface detection of S1PR1 in live intact human Jurkat T-cell leukemia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody (#ASR-011-F), (5 µg).

product information

CAT :

ASR-011-F

SKU :

ASR-011-F_50 mcl

Product Name :

Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21453

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR011

Homology :

Mouse, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1

Product Page - Scientific background :

Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4. Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult5.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CHEDG1, CD363

Short Description :

A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize S1PR1 from human, rat, and mouse samples. Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody (#ASR-011-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CHEDG1, CD363

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

S1PR1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASR-011), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage