Anti-Somatostatin Receptor Type 2 (extracellular) Antibody | Alomone Labs ASR-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Somatostatin Receptor Type 2 (extracellular) Antibody

catalog :

ASR-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Gao X, Abdelkarim H, Albee L, Volkman B, Gaponenko V, Majetschak M. Partial agonist activity of α1-adrenergic receptor antagonists for chemokine (C-X-C motif) receptor 4 and atypical chemokine receptor 3. PLoS ONE. 2018;13:e0204041 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and pancreas (lanes 2 and 4) lysates: - 1-2. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), (1:200).3-4. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody, preincubated with Somatostatin Receptor Type 2 (extracellular) Blocking Peptide (#BLP-SR006).

image 2 :

Expression of SSTR2 in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006). A. SSTR2 appears in neural processes (green) that are perpendicular to dentate granule layer (arrow). B. Staining of axons with mouse anti-Neurofilament 200 (NF200, red). C. Confocal merge of SSTR2 and NF200 images suggests that SSTR2 is not present in axons that run through the granule layer and hilus, but rather in neuronal dendrites that ascend toward the dentate hilus (arrow).

image 3 :

Western blot analysisof rat brain (lanes 1 and 3) and pancreas (lanes 2 and 4) lysates: - 1.Anti-Somatostatin Receptor Type2(extracellular)Antibody(#ASR-006) (1:200).2. Anti-Somatostatin Receptor Type2 (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

ASR-006

SKU :

ASR-006-CF_0.2 ml

Product Name :

Anti-Somatostatin Receptor Type 2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30680

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR006

Homology :

Mouse - identical; human- 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GSNQTEPYYDMTSN, corresponding to amino acid residues 30-43 of rat SSTR2

Product Page - Scientific background :

Somatostatin is a small cyclic peptide that is widely expressed throughout the central nervous system and peripheral tissues.1 In peripheral tissues, somatostatin exerts inhibitory effects on secretion processes, whereas in the brain, it acts as a neurotransmitter in both a stimulatory and an inhibitory manner.1,2Somatostatin mediates its action via six high affinity G-protein coupled receptors (SSTR1, SSTR2a, SSTR2b, SSTR3, SSTR4, and SSTR5), which are encoded by five genes.1,2 Expression of the different receptors is developmentally regulated in a time- and tissue-specific manner.2Somatostatin receptors have been found on a variety of neuroendocrine tumors, such as paragangliomas, carcinoids, and breast tumors.3 Synthetic peptide derivatives of somatostatin have been successfully used in the treatment of neuroendocrine malignancies and in vivo imaging of tumors that are positive for somatostatin receptors.4In general, SSTR2 is the most common SSTR subtype found in human tumors, followed by SSTR1, with SSTR3 and SSTR4 being less common.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

SSTR2, SS2R, Smstr2, SRIF1A

Short Description :

A Rabbit Polyclonal Antibody to Somatostatin Receptor Type 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against the N-terminal domain of the rat SSTR2. Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize SSTR2 from mouse, human and rat samples.

Negative Control :

BLP-SR006

Positive Control :

Synonyms :

SSTR2, SS2R, Smstr2, SRIF1A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SSTR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage