Anti-Somatostatin Receptor Type 5 (extracellular) Antibody | Alomone Labs ASR-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Somatostatin Receptor Type 5 (extracellular) Antibody

catalog :

ASR-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Lukomska A, Dobrzanski G, Liguz Lecznar M, Kossut M. Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex. Brain Struct Funct. 2020;225:387-401 pubmed publisher
Li X, Rao T, Xu Y, Hu K, Zhu Z, Li H, et al. Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury. Acta Pharmacol Sin. 2018;39:1373-1385 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005), (1:200).2. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody, preincubated with Somatostatin Receptor Type 5 (extracellular) Blocking Peptide (#BLP-SR005).

image 2 :

Expression of SSTR5 in mouse cerebellum - Immunohistochemical staining of Somatostatin receptor 5 (SSTR5) in mouse cerebellum using Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005). A. SSTR5 (red) appears in Purkinje cells (horizontal arrows) and in molecular layer interneurons (vertical arrows). B. Parvalbumin (green) appears in the Purkinje cells and their processes. C. merge of SSTR5 and parvalbumin demonstrates co-localization in Purkinje cells (horizontal arrows) but not in the molecular layer (vertical cells). DAPI is used as the counterstain (blue).

image 3 :

Expression of SSTR5 in human HT-29 cells - Cell surface detection ofSSTR5 in intact living human colorectal adenocarcinoma (HT-29) cells.A. Extracellular staining of cells withAnti-Somatostatin Receptor Type 5 (extracellular)Antibody (#ASR-005) (1:50) (red). B. Merged image of A with live view of cells.

product information

CAT :

ASR-005

SKU :

ASR-005-CF_0.2 ml

Product Name :

Anti-Somatostatin Receptor Type 5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30938

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR005

Homology :

Mouse - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CEPLSLASTPSWNAS, corresponding to amino acid residues 2-15 of rat SSTR5

Product Page - Scientific background :

Somatostatin (SST) is a small cyclic peptide that was first identified as a powerful inhibitor of the secretion of various hormones including growth hormone (GH), thyroid-stimulating hormone (TSH) and prolactin from the pituitary, as well as almost every major hormone from the intestinal tract. SST consists of two major bioactive forms, SST-14 and the N-terminus extended peptide SST-28, that can be produced by a wide variety of neuroendocrine, inflammatory and immune cells. In target cells, SST induces a variety of physiological functions that include neuromodulation, cell secretion, cell proliferation and smooth muscle contractility.SST acts on its multiple cell targets via a family of six receptors that originate from five genes: SSTR1, SSTR2a, SSTR2b, SSTRR3, SSTR4, SSTR5. The SSTRs are members of the G-protein coupled receptor superfamily and they modulate cell response via multiple second messenger systems such as inhibition of adenylate cyclase, modulation of conductance of ion channels and protein dephosphorylation.In contrast to SSTR1-4 that bind SST-14 and SST-28 with similar affinity, SSTR5 has a much greater affinity to SST-28 over SST-14.SSTRs are widely expressed in both the brain and peripheral tissues but with receptor subtype variations between the different cell types.Strong SSTR expression has been detected in a variety of human tumors including pancreatic, breast, lung and prostate cancers. In fact, SST has been shown to inhibit the growth of various normal and tumor cells. On this basis, several long lasting SST analogs have been developed and are being tested for use in several pathologies such as various cancers, acromegaly, immunoproliferative disorders, diabetic retinopathy, epilepsy and pain.SSTR5 is expressed in a variety of tissues and cells, including pancreas, colonic mucosa, small intestine, heart, and vascular smooth muscle cells.SSTR5 has been shown to mediate growth inhibition and cell proliferation, and to play an important role in cancer growth regulation as one of the most potent inhibitory receptors.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

SSTR5, SS5R, Smstr5, SST5

Short Description :

A Polyclonal Antibody to Somatostatin Receptor Type 5

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat SSTR5. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005), can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize SSTR5 in human, mouse and rat samples.

Negative Control :

BLP-SR005

Positive Control :

Synonyms :

SSTR5, SS5R, Smstr5, SST5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SSTR5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage