Anti-Somatostatin Receptor Type 4 (extracellular) Antibody | Alomone Labs ASR-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Somatostatin Receptor Type 4 (extracellular) Antibody

catalog :

ASR-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Lichvárová L, Henzi T, Safiulina D, Kaasik A, Schwaller B. Parvalbumin alters mitochondrial dynamics and affects cell morphology. Cell Mol Life Sci. 2018;75:4643-4666 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-Somatostatin Receptor Type 4 (extracellular) Antibody, (#ASR-004), (1:200).2. Anti-Somatostatin Receptor Type 4 (extracellular) Antibody, preincubated with Somatostatin Receptor Type 4 (extracellular) Blocking Peptide (#BLP-SR004).

image 2 :

Expression of SSTR4 in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-Somatostatin Receptor Type 4 (extracellular) Antibody, (#ASR-004). A. SSTR4 appears in the pyramidal layer (red). B. Staining of interneurons with mouse anti-parvalbumin (PV, green). C. Confocal merge of SSTR4 and PV demonstrates separate localization in hippocampus.

image 3 :

Expression of SSTR4 in rat hippocampus - Immunohistochemical staining of rat hippocampususingAnti-Somatostatin Receptor Type4 (extracellular)Antibody (#ASR-004). A. SSTR4 appears in the pyramidal layer (red). B. Staining of interneurons with mouse anti-parvalbumin (PV green). C. Confocal merge of SSTR4 and PV demonstrates separate localization in hippocampus.

product information

CAT :

ASR-004

SKU :

ASR-004-CF_0.2 ml

Product Name :

Anti-Somatostatin Receptor Type 4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30937

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR004

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

DTRPARGGEAVAC, corresponding to amino acid residues 182-194 of rat SSTR4

Product Page - Scientific background :

Somatostatin (SST) is a small cyclic peptide that was first identified as a powerful inhibitor of the secretion of various hormones including growth hormone (GH), thyroid-stimulating hormone (TSH) and prolactin from the pituitary, as well as almost every major hormone from the intestinal tract. SST consists of two major bioactive forms, SST-14 and the N-terminus extended peptide SST-28 that can be produced by a wide variety of neuroendocrine, inflammatory and immune cells. In target cells, SST induces a variety of physiological functions that include neuromodulation, cell secretion, cell proliferation and smooth muscle contractility.SST acts on its multiple cell targets via a family of six receptors that originate from five genes: SSTR1, SSTR2a, SSTR2b, SSTRR3, SSTR4, SSTR5. The SSTRs are members of the G-protein coupled receptor superfamily and they modulate cell responses via multiple second messenger systems such as inhibition of adenylate cyclase, modulation of conductance of ion channels and protein dephosphorylation.SSTRs are expressed widely in both the brain and peripheral tissues but with receptor subtype variations between the different cell types. SSTR4 is the SSTR subtype least expressed in the brain but is most strongly expressed in the lung, heart and placenta.Strong SSTR expression has been detected in a variety of human tumors including pancreatic, breast, lung and prostate cancers. In fact, SST has been shown to inhibit the growth of various normal and tumor cells. On this basis, several long lasting SST analogs have been developed and are being tested for use in several pathologies such as various cancers, acromegaly, immunoproliferative disorders, diabetic retinopathy, epilepsy and pain.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

SSTR4, SS4R, Smstr4

Short Description :

A Rabbit Polyclonal Antibody to Somatostatin Receptor Type 4

Long Description :

Anti-Somatostatin Receptor Type 4 (extracellular) Antibody (#ASR-004) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SSTR4 from rat, mouse, and human samples.

Negative Control :

BLP-SR004

Positive Control :

Synonyms :

SSTR4, SS4R, Smstr4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SSTR4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage