Anti-Somatostatin Receptor Type 3 Antibody | Alomone Labs ASR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Somatostatin Receptor Type 3 Antibody

catalog :

ASR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Lukomska A, Dobrzanski G, Liguz Lecznar M, Kossut M. Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex. Brain Struct Funct. 2020;225:387-401 pubmed publisher
Ye F, Nager A, Nachury M. BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone. J Cell Biol. 2018;217:1847-1868 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003), (1:200). 2. Anti-Somatostatin Receptor Type 3 Antibody, preincubated with Somatostatin Receptor Type 3 Blocking Peptide (#BLP-SR003).

image 2 :

Expression of SSTR3 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003). A. SSTR3 appears in Purkinje cells (red, arrow points at an example). B. Staining of Purkinje nerve cells with mouse anti parvalbumin (a calcium binding protein, green). C. Confocal merge of SSTR3 and parvalbumin demonstrates the co-localization of these proteins in Purkinje cells (arrow points at a Purkinje cell pointed at in A and B).

image 3 :

Peptide KVRSTTRRVRAPSC corresponding to amino acid residues 233-246 of rat SSTR3 (AccessionP30936). Intracellular betweentransmembrane domains 5and 6.

product information

CAT :

ASR-003

SKU :

ASR-003-CF_0.2 ml

Product Name :

Anti-Somatostatin Receptor Type 3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30936

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SR003

Homology :

Mouse - identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, between transmembrane domains 5 and 6

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

KVRSTTRRVRAPSC, corresponding to amino acid residues 233-246 of rat SSTR3

Product Page - Scientific background :

Somatostatin (SST) is a small cyclic peptide that was first identified as a powerful inhibitor of the secretion of various hormones including growth hormone (GH), thyroid-stimulating hormone (TSH) and prolactin from the pituitary, as well as practically every major hormone from the intestinal tract. SST consists of two major bioactive forms, SST-14 and the N-terminus extended peptide SST-28 that can be produced by a wide variety of neuroendocrine, inflammatory and immune cells. SST induces in target cells a variety of physiological functions that include nueromodulation, cell secretion, cell proliferation and smooth muscle contractility.SST acts on its multiple cell targets via a family of six receptors that are originated form five genes: SSTR1, SSTR2a, SSTR2b, SSTRR3, SSTR4, SSTR5. The SSTRs are members of the G-protein coupled receptor superfamily and they modulate cell response via multiple second messenger systems such as inhibition of adenylate cyclase, modulation of conductance of ion channels and protein dephosphorylation.SSTRs are expressed widely in both the brain and peripheral tissues but with receptor subtype variations between the different cell types. Thus, SSTR3 expression in the brain is primarily concentrated in the cerebellum with lesser expression in peripheral organs such as the spleen, the liver and the pituitary.Strong SSTR expression has been detected in a variety of human tumors including pancreatic, breast, lung and prostate cancers. In fact, SST has been shown to inhibit the growth of various normal and tumor cells. On this basis several long lasting SST analogs have been developed and are being tested for use in several pathologies such as various cancers, acromegaly, immunoproliferative disorders, diabetic retinopathy, epilepsy and pain.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

SSTR3, SS3R, Smstr3

Short Description :

A Rabbit Polyclonal Antibody to Somatostatin Receptor Type 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against a highly conserved epitope in the third intracellular loop of the rat SSTR3. Anti-Somatostatin Receptor Type 3 Antibody (#ASR-003) can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize SSTR3 from human, mouse, and rat samples.

Negative Control :

BLP-SR003

Positive Control :

Synonyms :

SSTR3, SS3R, Smstr3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SSTR3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage