Anti-SCN1A (NaV1.1) (extracellular) Antibody | Alomone Labs ASC-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN1A (NaV1.1) (extracellular) Antibody

catalog :

ASC-051

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):

image 2 :

Expression of Na v 1.1 in mouse cerebellum

image 3 :

Expression of Na v 1.1 in mouse cortex

product information

CAT :

ASC-051

SKU :

ASC-051-CF_0.2 ml

Product Name :

Anti-SCN1A (NaV1.1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

A2APX8

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC051

Homology :

Rat, human – identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EFDWKSYIQDSRYH, corresponding to amino acid residues 311 - 324 of mouse SCN1A

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3. Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,7. NaV1.1, also referred to as SCN1A, is a tetrodotoxin-sensitive channel and is broadly expressed in neurons7. Mutations in NaV1.1 are associated with at least two forms of epilepsy. Gain-of-function missense mutations are a primary cause of generalized epilepsy with febrile seizures plus (GEFS+). Loss-of-function mutations cause severe myoclonic epilepsy of infancy (SMEI)8,9.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Sodium channel protein type I subunit alpha, NAC1

Short Description :

A Rabbit polyclonal antibody to Nav1.1 (extracellular)

Long Description :

Anti-SCN1A (NaV1.1) (extracellular) Antibody (#ASC-051) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Nav1.1 from mouse, rat and human samples.

Negative Control :

BLP-SC051

Positive Control :

Synonyms :

Sodium channel protein type I subunit alpha, NAC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN1A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage