Anti-NaV1.5 (SCN5A) (extracellular) Antibody | Alomone Labs ASC-045 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.5 (SCN5A) (extracellular) Antibody

catalog :

ASC-045

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat heart lysate:

image 2 :

Western blot analysis of human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):

image 3 :

Cell surface detection of Nav1.5 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:

product information

CAT :

ASC-045

SKU :

ASC-045-CF_0.2 ml

Product Name :

Anti-NaV1.5 (SCN5A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JJV9

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC045

Homology :

Rat - identical, Human - 14 out of 16 residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 11th loop (3rd loop of 3rd repeat).

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CESFNVTGELYWTKVK, corresponding to amino acid residues 1386 - 1401 of mouse SCN5A

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.1 Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias.The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX)-insensitive NaV1.5.2The putative structure of NaV1.5 consists of four homologous domains (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome.1-2 Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system.1

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Sodium channel protein type 5 subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, Long QT Syndrome 3, LQT3

Short Description :

A Rabbit Polyclonal antibody to NaV1.5 (extracellular)

Long Description :

Anti-NaV1.5 (SCN5A) (extracellular) Antibody (#ASC-045) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human, and mouse samples.

Negative Control :

BLP-SC045

Positive Control :

Synonyms :

Sodium channel protein type 5 subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, Long QT Syndrome 3, LQT3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN5A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage