Anti-SCN4B (NaVβ4) (extracellular) Antibody | Alomone Labs ASC-044 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN4B (NaVβ4) (extracellular) Antibody

catalog :

ASC-044

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Xie W, Zhang J, Strong J, Zhang J. Role of NaV1.6 and NaVβ4 Sodium Channel Subunits in a Rat Model of Low Back Pain Induced by Compression of the Dorsal Root Ganglia. Neuroscience. 2019;402:51-65 pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 5), rat cortex (lane 2 and 6), mouse brain (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysates: - 1-4. Anti-SCN4B (NaVβ4) (extracellular) Antibody (#ASC-044), (1:800).5-8. Anti-SCN4B (NaVβ4) (extracellular) Antibody, preincubated with SCN4B/Navβ4 (extracellular) Blocking Peptide (#BLP-SC044).

image 2 :

Expression of NaVβ4 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-SCN4B (NaVβ4) (extracellular) Antibody (#ASC-044) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NaVβ4 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.

product information

CAT :

ASC-044

SKU :

ASC-044-CF_0.2 ml

Product Name :

Anti-SCN4B (NaVβ4) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7M730

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC044

Homology :

Mouse - identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β4 subunit of voltage-gated Na+ channels

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94,5. NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9) 2,3.Mammalian Na+ channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,7.NaVβ4 has been associated with Long QT syndrome8 and with Huntington's disease, showing a distinct down regulation of NaVβ4 in the presymptomatic stage of HD mice, whereas other voltage-gated ion channel subunits were later decreased9.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel subunit beta-4, LQT10

Short Description :

A Rabbit Polyclonal Antibody to NaVβ4 Subunit

Long Description :

Anti-SCN4B (NaVβ4) (extracellular) Antibody (#ASC-044) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting NaVβ4 in living cells. It has been designed to recognize NaVβ4 from rat, human, and mouse samples.

Negative Control :

BLP-SC044

Positive Control :

Synonyms :

Voltage-gated sodium channel subunit beta-4, LQT10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN4B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage