Anti-SCN1B (NaVβ1) (extracellular) Antibody | Alomone Labs ASC-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN1B (NaVβ1) (extracellular) Antibody

catalog :

ASC-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Helm M, Dankovich T, Mandad S, Rammner B, Jähne S, Salimi V, et al. A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines. Nat Neurosci. 2021;24:1151-1162 pubmed publisher
Richter K, Wildhagen H, Helm M, Ußling J, Schikorski T, Rizzoli S. Comparative synaptosome imaging: a semi-quantitative method to obtain copy numbers for synaptic and neuronal proteins. Sci Rep. 2018;8:14838 pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6): - 1-3. Anti-SCN1B (NaVβ1) (extracellular) Antibody (#ASC-041), (1:400).4-6. Anti-SCN1B (NaVβ1) (extracellular) Antibody, preincubated with SCN1B/Navβ1 (extracellular) Blocking Peptide (#BLP-SC041).

image 2 :

Expression of NaVβ1 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-SCN1B (NaVβ1) (extracellular) Antibody (#ASC-041) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. NaVβ1 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.

image 3 :

Expression of NaV?1in differentiated rat PC12 cells - Cell surface detection ofNaV?1in live and intact differentiated rat PC12 cells. PC12 differentiation was induced byNative mouse NGF 2.5S protein ( 95%)(#N-100). A C. Merge of NaV?1staining usingAnti-SCN1B (NaV?1)(extracellular)Antibody (#ASC-041) (1:50) (red) with live cell imaging. B D. Merge of NaV?1staining (1:50) (red) with nuclear staining using DAPI as the counterstain.

product information

CAT :

ASC-041

SKU :

ASC-041-CF_0.2 ml

Product Name :

Anti-SCN1B (NaVβ1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q00954

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC041

Homology :

Mouse - identical; human -13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CKRRSETTAETFTE, corresponding to amino acid residues 43-56 of rat β1 subunit of voltage-gated Na+ channels

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.2,3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β-subunits (NaV β-subunit).4The NaV β-subunit gene family consists of four members: β1 (SCN1B), β2 (SCN2B), β3 (SCN3B), and β4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus. They modulate channel gating, assembly, and cell surface expression in heterologous cell systems.5NaV β-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules.5In the adult central nervous system and heart, sodium channels are associate with β1-β4 subunits, whereas in adult skeletal muscle they are associate only with the β1 subunit.4,5 This association appears to be a late event in sodium channel biosynthesis.6

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel subunit beta-1, GEFSP1

Short Description :

A Rabbit Polyclonal Antibody to NaVβ1 Subunit

Long Description :

Anti-SCN1B (NaVβ1) (extracellular) Antibody (#ASC-041) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize NaVβ1 from human, rat, and mouse samples.

Negative Control :

BLP-SC041

Positive Control :

Synonyms :

Voltage-gated sodium channel subunit beta-1, GEFSP1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN1B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage