Anti-ASIC5 (extracellular) Antibody | Alomone Labs ASC-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASIC5 (extracellular) Antibody

catalog :

ASC-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat small intestine (lanes 3 and 6) lysates: - 1-3. Anti-ASIC5 (extracellular) Antibody (#ASC-032), (1:200).4-6. Anti-ASIC5 (extracellular) Antibody, preincubated with ASIC5 (extracellular) Blocking Peptide (#BLP-SC032).

image 2 :

Expression of ASIC5 in mouse cortex - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASIC5 (extracellular) Antibody (#ASC-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASIC5 immunoreactivity (green) is detected in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ASC-032

SKU :

ASC-032-CF_0.2 ml

Product Name :

Anti-ASIC5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9R0Y1

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC032

Homology :

Rat, human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RQLKTVHQEYPWGE, corresponding to amino acid residues 284-297 of mouse ASIC5

Product Page - Scientific background :

ASIC5 (acid sensing ion channel subunit family member 5) is an amiloride-sensitive ion channel belonging to the Degenerin/Epithelial Na+ channel (Deg/ENaC) family, a group of voltage-insensitive cation channels expressed in the nervous system and in several types of epithelial and immune cells.Structurally, ASICs have two transmembrane domains which line the pore of the channel with a relatively short cytoplasmic section, intracellular N- and C-termini and a large extracellular loop. The proton ligand binds to the extracellular domain, composed of 12 β sheets and 7 α helices2,3.ASICs are permeable to sodium, potassium, and sometimes to calcium and are activated by low pH, both intracellular and extracellular. They are responsible for conducting depolarizing inward Na+ currents. ASICs also contribute to excitotoxic neuronal damage during ischemia and local acidosis due to their sensitivity to acid1-3.ASICs are abundantly expressed in mammalian neurons. ASIC5 is mainly expressed in the brain in the cerebellum, specifically in the ventral uvula and nodulus of the vestibulocerebellum. ASIC5 mRNA is also detected in the liver mainly in the luminal membrane of epithelial cholangiocytes that line the bile duct1.ASICs have been linked to numerous neurological diseases including Parkinson's disease, stroke, traumatic brain injury, multiple sclerosis, and epilepsy3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5

Short Description :

A Rabbit Polyclonal Antibody to ASIC5 Channel

Long Description :

Anti-ASIC5 (extracellular) Antibody (#ASC-032) is a highly specific antibody directed against an epitope of the mouse protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC5 from human, mouse, and rat samples.

Negative Control :

BLP-SC032

Positive Control :

Synonyms :

Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage