Anti-pan ASIC (extracellular) Antibody | Alomone Labs ASC-031 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-pan ASIC (extracellular) Antibody

catalog :

ASC-031

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Zhao Y, Chen Y, Wang Z, Peng C, Li Q, Wu J, et al. The method of Yiqi Yangyin Tongluo can attenuate the pyroptosis of rheumatoid arthritis chondrocytes through the ASIC1a/NLRP3 signaling pathway. Ann Transl Med. 2022;10:145 pubmed publisher
Fila M, Sassi A, Brideau G, Cheval L, Morla L, Houillier P, et al. A variant of ASIC2 mediates sodium retention in nephrotic syndrome. JCI Insight. 2021;6: pubmed publisher
Xu X, Li Y, Wu Y, Xu Y, Weng R, Wang C, et al. Upregulation of spinal ASIC1 by miR-485 mediates enterodynia in adult offspring rats with prenatal maternal stress. CNS Neurosci Ther. 2021;27:244-255 pubmed publisher
Cai F, Hong X, Tang X, Liu N, Wang F, Zhu L, et al. ASIC1a activation induces calcium-dependent apoptosis of BMSCs under conditions that mimic the acidic microenvironment of the degenerated intervertebral disc. Biosci Rep. 2019;39: pubmed publisher
Dai B, Zhu F, Chen Y, Zhou R, Wang Z, Xie Y, et al. ASIC1a Promotes Acid-Induced Autophagy in Rat Articular Chondrocytes through the AMPK/FoxO3a Pathway. Int J Mol Sci. 2017;18: pubmed publisher
Zhou R, Dai B, Xie Y, Wu X, Wang Z, Li Y, et al. Interleukin-1β and tumor necrosis factor-α augment acidosis-induced rat articular chondrocyte apoptosis via nuclear factor-kappaB-dependent upregulation of ASIC1a channel. Biochim Biophys Acta Mol Basis Dis. 2018;1864:162-177 pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:500).3-4. Anti-pan ASIC (extracellular) Antibody, preincubated with Pan ASIC (extracellular) Blocking Peptide (#BLP-SC031).

image 2 :

Expression of ASIC channels in rat locus coeruleus - Immunohistochemical staining of perfusion-fixed rat brain frozen sections using Anti-pan ASIC (extracellular) Antibody (#ASC-031), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. ASIC channel staining (green) appears in neuronal soma (arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

ASC-031

SKU :

ASC-031-CF_0.2 ml

Product Name :

Anti-pan ASIC (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P55926

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC031

Homology :

ASIC1: Mouse, rat, human - identicalASIC2: Mouse, rat, human - 14/15 amino acid residues identicalASIC3: Mouse, rat, human - 12/15 amino acid residues identicalASIC4: Mouse, rat - 12/15 amino acid residues identical; human - 11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1

Product Page - Scientific background :

ASIC channels belong to the ENaC/Degenerin superfamily and are proton-gated ion channels which are not voltage-dependent. Six different genes encode the different ASIC subunits and splice variants: ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3 and ASIC4.ASIC channels sense protons and interact with proteins (namely proteases) and other ions such as Zn2+ and Ca2+ through their extracellular domains. The extracellular loop of ASIC1a undergoes cleavage by extracellular trypsin (a serine protease) which is responsible for shifting the pH dependence of the channel activation and inactivation to more acidic pH1.These channels are expressed in the brain and in the nervous system2 but have also been found in the retina, testes, and other tissues3,4 where they are involved in many physiological processes including nociception5, learning and memory6, mechanosensation7 among others.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4

Short Description :

A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)

Long Description :

Alomone Labs is pleased to offer an antibody directed against an epitope of rat ASIC1. The antibody recognizes ASIC1 through ASIC4. Anti-pan ASIC (extracellular) Antibody (#ASC-031) can be used in western blot, immunohistochemistry and immunocytochemistry in living cells. It has been designed to recognize all ASIC channels from rat, human and mouse samples.

Negative Control :

BLP-SC031

Positive Control :

Synonyms :

Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC2, ASIC1, ASIC3, ASIC4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage