Anti-ENaC α (SCNN1A) (extracellular) Antibody | Alomone Labs ASC-030 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ENaC α (SCNN1A) (extracellular) Antibody

catalog :

ASC-030

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Tong Y, Fang J, Song A, Deng H, Li P, Huang Z, et al. Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy. Ann Transl Med. 2022;10:242 pubmed publisher
Corrêa J, Boaro K, Sene L, Polidoro J, Salles T, Martins F, et al. Antiproteinuric and Hyperkalemic Mechanisms Activated by Dual Versus Single Blockade of the RAS in Renovascular Hypertensive Rats. Front Physiol. 2021;12:656460 pubmed publisher
Gruszczynska Biegala J, Strucinska K, Maciąg F, Majewski L, Sladowska M, Kuznicki J. STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons. Cells. 2020;9: pubmed publisher
Kemp B, Howell N, Gildea J, Padia S. Ghrelin-Induced Sodium Reabsorption Is Mediated by PKA and Microtubule-Dependent αENaC Translocation in Female Rats. J Endocr Soc. 2019;3:2088-2106 pubmed publisher
Ilatovskaya D, Levchenko V, Pavlov T, Isaeva E, Klemens C, Johnson J, et al. Salt-deficient diet exacerbates cystogenesis in ARPKD via epithelial sodium channel (ENaC). EBioMedicine. 2019;40:663-674 pubmed publisher
Quadri S, Culver S, Ramkumar N, Kohan D, Siragy H. (Pro)Renin receptor mediates obesity-induced antinatriuresis and elevated blood pressure via upregulation of the renal epithelial sodium channel. PLoS ONE. 2018;13:e0202419 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney lysates: - 1,2. Anti-ENaC α (SCNN1A) (extracellular) Antibody (#ASC-030), (1:200).3,4. Anti-ENaC α (SCNN1A) (extracellular) Antibody, preincubated with ENaC α/SCNN1A (extracellular) Blocking Peptide (#BLP-SC030).

image 2 :

Peptide (C)EELDRITEQTLFD corresponding to amino acid residues 173-185 of rat ENaC? (AccessionP37089). Extracellular loop.

image 3 :

Western blot analysis of rat brain membranes: - 1.Anti-LRRC26 Antibody (#APC-070) (1:200).2. Anti-LRRC26 Antibody preincubated with the negative control antigen.

product information

CAT :

ASC-030

SKU :

ASC-030-CF_0.2 ml

Product Name :

Anti-ENaC α (SCNN1A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P37089

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC030

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EELDRITEQTLFD, corresponding to amino acid residues 173-185 of rat ENaCα

Product Page - Scientific background :

The DEG/ENaC family consists of Na+ selective channels. They are characterized by 2 transmembrane domains with a large extracellular loop, important for channel activity and intracellular N-and C-termini which are important for channel gating and for interacting with regulatory proteins respectively1,2.ENaC is localized to the apical side of polarized epithelial cells like those in the kidney, lung, and distal colon and is responsible for maintaining proper Na+ balance. This channel is formed by oligomerization of three subunits, α, β, and γ usually with the α2β1γ1 combination2,3. In addition, the α subunit which is essential for channel activity undergoes phosphorylation in its C-terminal by SGK1 (serum and glucocorticoid-induced kinase 1) which is known to activate the channel at the plasma membrane4.Pseudohypoaldosteronism type 1 (PHA-1) has an early onset and is depicted by severe dehydration, hyponatremia and hyperkalemia5. One form of the pathology is characterized by mutations in α, β, and γ subunits5. The severity of the diseases depends on the loss of activity of the channel2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial sodium channel α subunit, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA

Short Description :

A Rabbit Polyclonal Antibody to ENaCα Channel

Long Description :

Anti-ENaC α (SCNN1A) (extracellular) Antibody (#ASC-030) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular domain and can potentially detect the channel in living cells. It has been designed to recognize ENaCα from human, rat, and mouse samples.

Negative Control :

BLP-SC030

Positive Control :

Synonyms :

Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial sodium channel α subunit, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCNN1A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage