Anti-Human NaV1.7 (SCN9A) (extracellular) Antibody | Alomone Labs ASC-029 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human NaV1.7 (SCN9A) (extracellular) Antibody

catalog :

ASC-029

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human Caco-2 colorectal adenocarcinoma cell line lysate (lanes 1 and 3) and human U-87 MG glioma cell line lysate (lanes 2 and 4):

image 2 :

Cell surface detection of Na V 1.7 by indirect flow cytometry in live intact human U-87 MG glioma cell line:

image 3 :

Expression of Na V 1.7 in live intact human U-87 MG glioma cell line

product information

CAT :

ASC-029

SKU :

ASC-029-CF_0.2 ml

Product Name :

Anti-Human NaV1.7 (SCN9A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q15858

Applications :

IC IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-SC029

Homology :

Human only Not recommended for rat or mouse samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 11th loop (3rd loop of 3rd repeat).

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)DGSRFPASQVPNRSE, corresponding to amino acid residues 1355 - 1369 of human SCN9A

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

Applications may also work in :

IC IFC LCI WB

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel type IX subunit alpha, PN1

Short Description :

A Rabbit Polyclonal Antibody to Human NaV1.7 (SCN9A)

Long Description :

Anti-Human NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-029) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunocytochemistry and flow cytometry applications. It has been designed to recognize the human NaV1.7 protein. Not recommended for rat or mouse samples.

Negative Control :

BLP-SC029

Positive Control :

Synonyms :

Voltage-gated sodium channel type IX subunit alpha, PN1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN9A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage