Anti-NaV1.8 (SCN10A) (extracellular) Antibody | Alomone Labs ASC-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.8 (SCN10A) (extracellular) Antibody

catalog :

ASC-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates: - 1,2. Anti-NaV1.8 (SCN10A) (extracellular) Antibody (#ASC-028), (1:200).3,4. Anti-NaV1.8 (SCN10A) (extracellular) Antibody, preincubated with Nav1.8/SCN10A (extracellular) Blocking Peptide (#BLP-SC028).

image 2 :

Peptide (C)EDEVAAKEGNSPGPQ corresponding to amino acid residues 1943-1956 of rat NaV1.8 (AccessionQ63554). Intracellular C-terminus.

image 3 :

Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates: - 12.Anti-NaV1.8 (SCN10A) (extracellular)Antibody (#ASC-028) (1:200).34. Anti-NaV1.8 (SCN10A) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

ASC-028

SKU :

ASC-028-CF_0.2 ml

Product Name :

Anti-NaV1.8 (SCN10A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62968

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC028

Homology :

Mouse - identical; human - Not recommended for use with human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop, domain III.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDNYLEEKPRVKSVLE, corresponding to amino acid residues 1173-1188 of rat NaV1.8

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5b Expression of the α subunit isoform is developmentally and tissue specific.Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.6

Applications may also work in :

Supplier :

Alomone Labs

Target :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Short Description :

A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat NaV1.8 channel. Anti-NaV1.8 (SCN10A) (extracellular) Antibody (#ASC-028) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting NaV1.8 in living cells. It has been designed to recognize NaV1.8 from rat and mouse samples. The antibody will not recognize NaV1.8 from human samples.

Negative Control :

BLP-SC028

Positive Control :

Synonyms :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN10A

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage