Anti-NaV1.7 (SCN9A) (extracellular) Antibody | Alomone Labs ASC-027 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.7 (SCN9A) (extracellular) Antibody

catalog :

ASC-027

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
François Moutal L, Dustrude E, Wang Y, Brustovetsky T, Dorame A, Ju W, et al. Inhibition of the Ubc9 E2 SUMO-conjugating enzyme-CRMP2 interaction decreases NaV1.7 currents and reverses experimental neuropathic pain. Pain. 2018;159:2115-2127 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and rat DRG (lanes 2 and 4) lysates: - 1,2. Anti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027), (1:200).3,4. Anti-NaV1.7 (SCN9A) (extracellular) Antibody, preincubated with Nav1.7/SCN9A (extracellular) Blocking Peptide (#BLP-SC027).

image 2 :

Peptide (C)EKEGQTEYMDY(K) corresponding to amino acid residues 1533-1543 of rat NaV1.7 (AccessionO08562). 1stextracellular loop domain IV.

image 3 :

Expression of NaV1.7in rat PC12 cells - Cell surface detection ofNaV1.7 in intact living ratpheochromocytomaPC12 cells.A. Extracellular staining of cellsusingAnti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027) (1:50) followed by goat anti-rabbit-AlexaFluor-594secondary antibody(red). B. Merge image of A and live view of the cells.

product information

CAT :

ASC-027

SKU :

ASC-027-CF_0.2 ml

Product Name :

Anti-NaV1.7 (SCN9A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08562

Applications :

IC IF LCI WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC027

Homology :

Mouse - 8/11 amino acid residues identicalHuman - Not recommended to use with human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop, domain IV.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EKEGQTEYMDY(K), corresponding to amino acid residues 1533-1543 of rat NaV1.7

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Short Description :

A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat NaV1.7 channel. Anti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027) can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and thus is ideal for detecting NaV1.7 in living cells. It has been designed to recognize NaV1.7 from rat and mouse samples. The antibody will not recognize NaV1.7 from human samples.

Negative Control :

BLP-SC027

Positive Control :

Synonyms :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN9A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage