Anti-SCN3A (NaV1.3) (extracellular) Antibody | Alomone Labs ASC-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN3A (NaV1.3) (extracellular) Antibody

catalog :

ASC-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat newborn brain lysates (lanes 1 and 3), rat adult brain membranes (lane 2): - 1,2. Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023), (1:200).3. Anti-SCN3A (NaV1.3) (extracellular) Antibody, preincubated with SCN3A/Nav1.3 (extracellular) Blocking Peptide (#BLP-SC023).

product information

CAT :

ASC-023

SKU :

ASC-023-CF_0.2 ml

Product Name :

Anti-SCN3A (NaV1.3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P08104

Applications :

IC IF LCI WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC023

Homology :

Mouse, human - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop, domain I.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7 NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Short Description :

A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat NaV1.3 channel. Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023) can be used in western blot, and live cell imaging applications. It has been designed to recognize NaV1.3 from rat, human and mouse samples. The antibody may recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Negative Control :

BLP-SC023

Positive Control :

Synonyms :

BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN3A

AB Specifictiy :

May recognize NaV1.1 and NaV1.2 due to a moderate homology between the chosen sequence and the sequences of NaV1.1 and NaV1.2 (although there are no five amino acids identical in a row as considered to be the minimum needed for antibody/antigen recognition).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC FC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage