Anti-NALCN/VGCNL1 (extracellular) Antibody | Alomone Labs ASC-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NALCN/VGCNL1 (extracellular) Antibody

catalog :

ASC-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Um K, Hahn S, Kim S, Lee Y, Birnbaumer L, Kim H, et al. TRPC3 and NALCN channels drive pacemaking in substantia nigra dopaminergic neurons. elife. 2021;10: pubmed publisher
Sun L, Xia R, Jiang J, Wen T, Huang Z, Qian R, et al. MicroRNA-96 is required to prevent allodynia by repressing voltage-gated sodium channels in spinal cord. Prog Neurobiol. 2021;202:102024 pubmed publisher
Hahn S, Kim S, Um K, Kim H, Park M. N-benzhydryl quinuclidine compounds are a potent and Src kinase-independent inhibitor of NALCN channels. Br J Pharmacol. 2020;: pubmed publisher
Leo M, Schmitt L, Jastrow H, Thomale J, Kleinschnitz C, Hagenacker T. Cisplatin alters the function and expression of N-type voltage-gated calcium channels in the absence of morphological damage of sensory neurons. Mol Pain. 2017;13:1744806917746565 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and human SH-SYS5 brain neuroblastoma cell (lanes 3 and 6) lysates: - 1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody, preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).

image 2 :

Expression of NALCN in rat hypothalamus - Immunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.

image 3 :

Expression ofNALCN in rat PC12 cells - Cell surface detection ofNALCN in intact living rat pheochromocytoma PC12 cells. Extracellular staining of cells usingAnti-NALCN/VGCNL1 (extracellular) Antibody(#ASC-022) (1:50) followed by goat anti-rabbit-AlexaFluor-594secondary antibody. Merged image of extracellular staining with live image of the cells.

product information

CAT :

ASC-022

SKU :

ASC-022-CF_0.2 ml

Product Name :

Anti-NALCN/VGCNL1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6Q760

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC022

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, S1-S2 domain III.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN

Product Page - Scientific background :

The existence of resting K+ conductance has been known for some time. Only recently has the Nalcn (sodium leak channel non-selective protein) been found responsible for the resting Na+ conductance1.Nalcn is a member of the NaV and CaV channel family which have four homologous repeats of six transmembrane domains. One structural difference is that Nalcn is less positively charged in the voltage sensing segment1. The protein forms voltage-independent non-inactivating channel which is permeable to Na+, K+ and Ca2+ ions1,2.Evidence suggests that Nalcn is a non-redundant and thus essential channel since knock-out mice display disrupted respiratory rhythm and die within 24 hours of birth. In addition, isolated neurons from these mice have decreased excitability1.The channel is extensively expressed in the central nervous system1, heart, pituitary and adrenal glands and pancreatic islet cells2,3.In pancreatic islets, Nalcn is activated by acetylcholine via its physical association with M3 muscarinic receptor, and potentiates insulin secretion induced by glucose uptake2-4.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel

Short Description :

A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)

Long Description :

Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022) is a highly specific antibody directed against an epitope of rat Sodium leak channel non-selective protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NALCN from mouse, rat, and human samples.

Negative Control :

BLP-SC022

Positive Control :

Synonyms :

Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NALCN

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage