Anti-SCN4A (NaV1.4) Antibody | Alomone Labs ASC-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN4A (NaV1.4) Antibody

catalog :

ASC-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Kwan H, Chan Z, Bi X, Kutkowska J, Pr xf3 szy x144 ski T, Chan C, et al. Nerve-independent formation of membrane infoldings at topologically complex postsynaptic apparatus by caveolin-3. Sci Adv. 2023;9:eadg0183 pubmed publisher
Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
Banks Q, Pratt S, Iyer S, Lovering R, Hernández Ochoa E, Schneider M. Optical Recording of Action Potential Initiation and Propagation in Mouse Skeletal Muscle Fibers. Biophys J. 2018;115:2127-2140 pubmed publisher
Ghovanloo M, Abdelsayed M, Peters C, Ruben P. A Mixed Periodic Paralysis & Myotonia Mutant, P1158S, Imparts pH-Sensitivity in Skeletal Muscle Voltage-gated Sodium Channels. Sci Rep. 2018;8:6304 pubmed publisher

image

image 1 :

Western blot analysis of rat skeletal muscle lysate: - 1. Anti-SCN4A (NaV1.4) Antibody (#ASC-020), (1:200).2. Anti-SCN4A (NaV1.4) Antibody, preincubated with SCN4A/Nav1.4 Blocking Peptide (#BLP-SC020).

image 2 :

Peptide EDKELKDNHILNHVG(C) corresponding to amino acid residues 877-891 of rat NaV1.4 (AccessionP15390). Intracellular loop between domains II and III.

image 3 :

Alomone Labs Gabazine inhibits GABA(A) receptors expressed inXenopusoocytes. - Time course of GABA(A) ?1/?2 currents activated by a continuous application (top dotted line) of 0.1 M?-Aminobutyric acid(#G-110) and inhibited by 1 MGabazine(#G-215) as indicated while membrane potential was held at -60 mV.

product information

CAT :

ASC-020

SKU :

ASC-020-CF_0.2 ml

Product Name :

Anti-SCN4A (NaV1.4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15390

Applications :

IC IF WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC020

Homology :

Mouse - 15/17 amino acid residues identical; human - 14/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains II and III

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

EDKELKDNHILNHVG(C), corresponding to amino acid residues 877-891 of rat NaV1.4

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6The isoform NaV1.4, is primarily expressed in skeletal muscle.7 Different missense mutations in the gene for the NaV1.4 are correlated with several muscular diseases such as Paramyotonia congenital (PMC), PMC without cold paralysis, potassium-aggravating myotonia, and hyperkalemic periodic paralysis.8

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

Sodium channel protein type 4 subunit α, SkM1, Sodium channel protein skeletal muscle subunit α, HYKPP

Short Description :

A Rabbit Polyclonal Antibody to SCN4A (NaV1.4) Channel

Long Description :

Anti-SCN4A (NaV1.4) Antibody (#ASC-020) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize NaV1.4 from rat, human, and mouse samples.

Negative Control :

BLP-SC020

Positive Control :

Synonyms :

Sodium channel protein type 4 subunit α, SkM1, Sodium channel protein skeletal muscle subunit α, HYKPP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN4A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage