Anti-ENaC β (SCNN1B) (extracellular) Antibody | Alomone Labs ASC-019 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ENaC β (SCNN1B) (extracellular) Antibody

catalog :

ASC-019

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig 2a western blot; mouse; loading ...; fig 1b western blot; human; fig 3a	Garcia Caballero A, Gandini M, Huang S, Chen L, Souza I, Dang Y, et al. Cav3.2 calcium channel interactions with the epithelial sodium channel ENaC. Mol Brain. 2019;12:12 pubmed publisher
	Sung C, Chen M, Lin Y, Lin Y, Lin Y, Yang S, et al. Urinary Extracellular Vesicles for Renal Tubular Transporters Expression in Patients With Gitelman Syndrome. Front Med (Lausanne). 2021;8:679171 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and lung (lanes 2 and 4) lysates: - 1,2. Anti-ENaC β (SCNN1B) (extracellular) Antibody (#ASC-019), (1:200).3,4. Anti-ENaC β (SCNN1B) (extracellular) Antibody, preincubated with ENaC β/SCNN1B (extracellular) Blocking Peptide (#BLP-SC019).

image 2 :

Western blot analysisof rat kidney (lanes 1 and 3) and lung (lanes 2 and 4) lysates: - 12.Anti-ENaC ? (SCNN1B) (extracellular) Antibody(#ASC-019) (1:200).34. Anti-ENaC ? (SCNN1B) (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

ASC-019

SKU :

ASC-019-CF_0.2 ml

Product Name :

Anti-ENaC β (SCNN1B) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P37090

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC019

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

EFNYRTIEESPANNI(C), corresponding to amino acid residues 498-513 of rat ENaCβ

Product Page - Scientific background :

The amiloride-sensitive epithelial Na+ channel (ENaC) family includes 4 members: ENaCα, β, γ and δ. The ENaC subunits have a conserved topology consisting of two membrane-spanning domains with intracellular N and C-termini and a large glycosylated extracellular region.The functional ENaC channel is a heteromer with a presumed stoichiometry of α2βγ while the δ subunit can substitute for the α subunit in some tissues. Interestingly, neither the β nor the γ subunits are capable of producing any current when expressed alone in heterologous systems, while in these systems the simultaneous presence of all three ENaC subunits will produce Na+ currents that resemble the endogenous channel. The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin where it enables entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance as well as liquid balance in the lung. The central role of ENaC in the regulation of Na+ homeostasis and hence blood pressure is underscored by the identification of two human diseases that arise from either gain- or loss-of-function mutations of the ENaC channel. Liddle's syndrome is an inherited form of hypertension that stems from a dominant mutation of the ENaC channel (in either the β or γ subunits) that results in excessive activity of the channel and hence increased Na+ absorption. Conversely, pseudoaldosteronism type I (PHA) is a dysfunction characterized by hypotension due to poor Na+ absorption that is associated with loss-of-function mutations which may occur in each of the three ENaC subunits.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial sodium channel β subunit, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB

Short Description :

A Rabbit Polyclonal Antibody to ENaCβ Channel

Long Description :

Anti-ENaC β (SCNN1B) (extracellular) Antibody (#ASC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ENaCβ from rat, human, and mouse samples.

Negative Control :

BLP-SC019

Positive Control :

Synonyms :

Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial sodium channel β subunit, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCNN1B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage