Anti-ASIC3 Antibody | Alomone Labs ASC-018 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASIC3 Antibody

catalog :

ASC-018

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; fig 2c western blot; rat; 1:200; fig 2a	Hu Y, Mi X, Huang C, Wang H, Song J, Shu Q, et al. Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes. Sci Rep. 2017;7:44951 pubmed publisher
	Ding J, Zhang R, Li H, Ji Q, Cheng X, Thorne R, et al. ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration. Aging (Albany NY). 2021;13:10703-10723 pubmed publisher
	Niibori M, Kudo Y, Hayakawa T, Ikoma Seki K, Kawamata R, Sato A, et al. Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats. Heliyon. 2020;6:e03963 pubmed publisher
	Yang J, Qiu L, Strobel M, Kabel A, Zha X, Chen X. Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid Sensing of Mouse Olfactory Sensory Neurons. Mol Neurobiol. 2020;57:3042-3056 pubmed publisher
	Inprasit C, Lin Y. TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model. Int J Mol Sci. 2020;21: pubmed publisher
	Stephan G, Huang L, Tang Y, Vilotti S, Fabbretti E, Yu Y, et al. The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nat Commun. 2018;9:1354 pubmed publisher
	Hsieh W, Kung C, Huang S, Lin S, Sun W. TDAG8, TRPV1, and ASIC3 involved in establishing hyperalgesic priming in experimental rheumatoid arthritis. Sci Rep. 2017;7:8870 pubmed publisher
	Wu Y, Qin D, Yang H, Fu H. Evidence for the Participation of Acid-Sensing Ion Channels (ASICs) in the Antinociceptive Effect of Curcumin in a Formalin-Induced Orofacial Inflammatory Model. Cell Mol Neurobiol. 2017;37:635-642 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysate: - 1. Anti-ASIC3 Antibody (#ASC-018), (1:200).2. Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).

image 2 :

Multiplex staining of ASIC1 and ASIC3 in rat DRG - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:400) and rabbit Anti-ASIC3 Antibody (#ASC-018), (1:400). A. ASIC3 staining. B. ASIC1 staining in same section (green). C. Merge of the two images shows some co-localization (arrow). Cell nuclei were stained with DAPI (blue).

image 3 :

Western blot analysisof rat DRG lysate: - 1.Anti-ASIC3Antibody (#ASC-018)(1:200).2. Anti-ASIC3 Antibody preincubated with the negative control antigen.

product information

CAT :

ASC-018

SKU :

ASC-018-CF_0.2 ml

Product Name :

Anti-ASIC3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35240

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC018

Homology :

Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3

Product Page - Scientific background :

ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Short Description :

A Rabbit Polyclonal Antibody to ASIC3 Channel

Long Description :

Anti-ASIC3 Antibody (#ASC-018) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples.

Negative Control :

BLP-SC018

Positive Control :

Synonyms :

Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage