antibody

Guinea pig Anti-ASIC3 Antibody

ASC-018-GP

polyclonal

guinea-pigs

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry, immunocytochemistry

ASC-018-GP-CF_0.2 ml

Guinea pig Anti-ASIC3 Antibody

Antibodies

Antibodies

Polyclonal

O35240

IF IHC WB

Rat Mouse

Guinea Pig

BLP-SC018

Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical

PBS pH7.4

Guinea pig total IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3

ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC2, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

IF IHC WB

Alomone Labs

Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

A Guinea Pig Polyclonal Antibody to ASIC3 Channel

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat ASIC3. Guinea pig Anti-ASIC3 Antibody (#ASC-018-GP, formerly AGP-052) raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-ASIC3 Antibody (#ASC-018) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

BLP-SC018

NA

Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

ASIC3

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

ICC IHC

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:1000