Anti-SCN11A (NaV1.9) Antibody | Alomone Labs ASC-017 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN11A (NaV1.9) Antibody

catalog :

ASC-017

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Vincent K, Mohanty S, Pinelli R, Bonavita R, Pricop P, Albert T, et al. Aging of mouse intervertebral disc and association with back pain. Bone. 2019;123:246-259 pubmed publisher
Salvatierra J, Diaz Bustamante M, Meixiong J, Tierney E, Dong X, Bosmans F. A disease mutation reveals a role for NaV1.9 in acute itch. J Clin Invest. 2018;128:5434-5447 pubmed publisher
Gawlak M, Szulczyk B, Berłowski A, Grzelka K, Stachurska A, Pełka J, et al. Age-dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats. Dev Neurobiol. 2017;77:1371-1384 pubmed publisher
Nencini S, Ringuet M, Kim D, Chen Y, Greenhill C, Ivanusic J. Mechanisms of nerve growth factor signaling in bone nociceptors and in an animal model of inflammatory bone pain. Mol Pain. 2017;13:1744806917697011 pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysates: - 1. Anti-SCN11A (NaV1.9) Antibody (#ASC-017), (1:200).2. Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).

image 2 :

Expression of NaV1.9 in rat embryo DRG - Immunohistochemical staining of formalin frozen rat embryo dorsal root ganglion (DRG) sections with Anti-SCN11A (NaV1.9) Antibody (#ASC-017). A. Cells within the DRG were stained (see solid line frame enlarged in B) as well as fibers and the area of entry of dorsal root into the spinal cord (see dashed line frame enlarged in C). DAPI is used as the counterstain.

image 3 :

Peptide CNGDLSSLDVAKVKVHND corresponding to amino acid residues 1748-1765 of rat NaV1.9 (AccessionO88457). Intracellular C-terminus.

product information

CAT :

ASC-017

SKU :

ASC-017-CF_0.2 ml

Product Name :

Anti-SCN11A (NaV1.9) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88457

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC017

Homology :

Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 Expression of the α subunit is developmentally and tissue specific.The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons.4-5Recently, it was shown that BDNF activates NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone.6

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Short Description :

A Rabbit Polyclonal Antibody to SCN11A (NaV1.9) Channel

Long Description :

Anti-SCN11A (NaV1.9) Antibody (#ASC-017) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize NaV1.9 channel from rat, human, and mouse samples.

Negative Control :

BLP-SC017

Positive Control :

Synonyms :

PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN11A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage