Guinea pig Anti-SCN11A (NaV1.9) Antibody | Alomone Labs ASC-017-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-SCN11A (NaV1.9) Antibody

catalog :

ASC-017-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Zu M, Guo W, Cong T, Ji F, Zhang S, Zhang Y, et al. SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves. BMC Neurosci. 2021;22:18 pubmed publisher
Gawlak M, Szulczyk B, Berłowski A, Grzelka K, Stachurska A, Pełka J, et al. Age-dependent expression of Nav1.9 channels in medial prefrontal cortex pyramidal neurons in rats. Dev Neurobiol. 2017;77:1371-1384 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane: - 1. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#ASC-017-GP), (1:200).2. Guinea pig Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).

image 2 :

Expression of NaV1.9 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-SCN11A (NaV1.9) Antibody (#ASC-017-GP). A, D. NaV1.9 staining (green) appears in DRG neurons. B, E. Nuclear staining using DAPI as the counterstain (blue). C, F. Merge images of A, B and E, F.

product information

CAT :

ASC-017-GP

SKU :

ASC-017-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-SCN11A (NaV1.9) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88457

Applications :

IF IHC WB

Reactivity :

Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-SC017

Homology :

Mouse - 15/18 amino acid residues identical; human - 14/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. Their expression is developmentally and tissue specific.NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons4,5.Recently, it has been shown that BDNF activated NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Short Description :

A Guinea Pig Polyclonal Antibody to SCN11A (NaV1.9) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat protein. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#ASC-017-GP, formerly AGP-030), raised in guinea pigs, can be used in western blot and immunohistochemical applications, and has been designed to recognize NaV1.9 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-SCN11A (NaV1.9) Antibody (#ASC-017) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-SC017

Positive Control :

Synonyms :

PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN11A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage