Anti-NaV1.8 (SCN10A) Antibody | Alomone Labs ASC-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.8 (SCN10A) Antibody

catalog :

ASC-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat; 1:200; loading ...; fig 4d	Lin W, Zhang W, Lyu N, Cao H, Xu W, Zhang Y. Growth Differentiation Factor-15 Produces Analgesia by Inhibiting Tetrodotoxin-Resistant Nav1.8 Sodium Channel Activity in Rat Primary Sensory Neurons. Neurosci Bull. 2021;37:1289-1302 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 1b	Zhu J, Zhen G, An S, Wang X, Wan M, Li Y, et al. Aberrant subchondral osteoblastic metabolism modifies NaV1.8 for osteoarthritis. elife. 2020;9: pubmed publisher
	Alich T, R xf6 derer P, Szalontai B, Golcuk K, Tariq S, Peitz M, et al. Bringing to light the physiological and pathological firing patterns of human induced pluripotent stem cell-derived neurons using optical recordings. Front Cell Neurosci. 2022;16:1039957 pubmed publisher
	Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
	Qu R, Yao F, Zhang X, Gao Y, Liu T, Hua Y. SMN deficiency causes pain hypersensitivity in a mild SMA mouse model through enhancing excitability of nociceptive dorsal root ganglion neurons. Sci Rep. 2019;9:6493 pubmed publisher
	Meents J, Bressan E, Sontag S, Foerster A, Hautvast P, R xf6 sseler C, et al. The role of Nav1.7 in human nociceptors: insights from human induced pluripotent stem cell-derived sensory neurons of erythromelalgia patients. Pain. 2019;160:1327-1341 pubmed publisher
	Sun Y, Yang P, Song Z, Feng Y, Hu D, Hu J, et al. α-lipoic acid suppresses neuronal excitability and attenuates colonic hypersensitivity to colorectal distention in diabetic rats. J Pain Res. 2017;10:1645-1655 pubmed publisher
	Yang K, Kim M, Ju J, Park S, Lee C, Kim S, et al. Antinociceptive Effects of Botulinum Toxin Type A on Trigeminal Neuropathic Pain. J Dent Res. 2016;95:1183-90 pubmed publisher
	Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher
	Yan J, Zou K, Liu X, Hu S, Wang Q, Miao X, et al. Hyperexcitability and sensitization of sodium channels of dorsal root ganglion neurons in a rat model of lumber disc herniation. Eur Spine J. 2016;25:177-85 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysates: - 1. Anti-NaV1.8 (SCN10A) Antibody (#ASC-016), (1:200).2. Anti-NaV1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).

image 2 :

Expression of NaV1.8 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-NaV1.8 (SCN10A) Antibody (#ASC-016). NaV1.8 staining (red) is cytoplasmic and the intensity varies among DRG cells. There is a partial overlap in the distribution of NaV1.8 and neurofilament 200 (green).

product information

CAT :

ASC-016

SKU :

ASC-016-CF_0.2 ml

Product Name :

Anti-NaV1.8 (SCN10A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63554

Applications :

IC IF IHC IP WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC016

Homology :

Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 Expression of the α subunit isoform is developmentally and tissue specific.Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.6

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Short Description :

A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel

Long Description :

Anti-NaV1.8 (SCN10A) Antibody (#ASC-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize NaV1.8 from rat and mouse samples.

Negative Control :

BLP-SC016

Positive Control :

Synonyms :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN10A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage