Guinea pig Anti-NaV1.8 (SCN10A) Antibody | Alomone Labs ASC-016-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-NaV1.8 (SCN10A) Antibody

catalog :

ASC-016-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Vincent K, Mohanty S, Pinelli R, Bonavita R, Pricop P, Albert T, et al. Aging of mouse intervertebral disc and association with back pain. Bone. 2019;123:246-259 pubmed publisher
Nencini S, Ringuet M, Kim D, Chen Y, Greenhill C, Ivanusic J. Mechanisms of nerve growth factor signaling in bone nociceptors and in an animal model of inflammatory bone pain. Mol Pain. 2017;13:1744806917697011 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysate: - 1. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP), (1:200).2. Guinea pig Anti-NaV1.8 (SCN10A) Antibody, preincubated with Nav1.8/SCN10A Blocking Peptide (#BLP-SC016).

image 2 :

Expression of NaV1.8 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP). A. NaV1.8 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.

product information

CAT :

ASC-016-GP

SKU :

ASC-016-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-NaV1.8 (SCN10A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63554

Applications :

IF IHC WB

Reactivity :

Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-SC016

Homology :

Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. The expression of the α subunit isoform is developmentally regulated and tissue specific.Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons4-6. TTX-resistant channels have been suggested to play an important role in nociceptive transmission.Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models6.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Short Description :

A Guinea Pig Polyclonal Antibody to NaV1.8 (SCN10A) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat NaV1.8 channel. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP, formerly AGP-029), raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaV1.8 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.8 (SCN10A) Antibody (#ASC-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-SC016

Positive Control :

Synonyms :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN10A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage