Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody | Alomone Labs ASC-016-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody

catalog :

ASC-016-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Qu R, Yao F, Zhang X, Gao Y, Liu T, Hua Y. SMN deficiency causes pain hypersensitivity in a mild SMA mouse model through enhancing excitability of nociceptive dorsal root ganglion neurons. Sci Rep. 2019;9:6493 pubmed publisher

image

image 1 :

Expression of NaV1.8 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody (#ASC-016-AR). A. NaV1.8 labeling (red) appears in the cell bodies of the DRG. Note that the nerve fibers are not stained. B. Nuclear staining using DAPI as counter stain. C. Merged image of A and B.

image 2 :

Expression of NaV1.8 in rat DRG - Immunohistochemical staining of adult rat dorsal root ganglion (DRG) usingAnti-NaV1.8 (SCN10A)-ATTO-594Antibody (#ASC-016-AR). A. NaV1.8 labeling (red) appears in the cell bodies of the DRG. Note that the nerve fibers are not stained. B. Nuclear staining using DAPI as counter stain. C. Merged image of A and B.

image 3 :

Colocalization of NaV1.8 and Synaptophysin in rat DRG - Immunohistochemical staining of rat DRG frozen section usingAnti-NaV1.8 (SCN10A)-ATTO-594Antibody (#ASC-016-AR) andAnti-SynaptophysinAntibody (#ANR-013). A. NaV1.8 staining (red). B. SYP staining (green). C. Merged image demonstrates a partial overlap in the distribution of NaV1.8 and SYP within the DRGs. DAPI is used as the counterstain (blue).

product information

CAT :

ASC-016-AR

SKU :

ASC-016-AR_50 mcl

Product Name :

Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63554

Applications :

IF IHC

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC016

Homology :

Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. Their expression is developmentally regulated and tissue specific.Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons4,6. TTX-resistant channels have been suggested to play an important role in nociceptive transmission.Recently, the involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models6.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Short Description :

A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-NaV1.8 (SCN10A) Antibody (#ASC-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize NaV1.8 from rat, human, and mouse samples. Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody (#ASC-016-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-SC016

Positive Control :

Synonyms :

PN3, SNS, Sodium channel protein type 10 subunit alpha

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN10A

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASC-016), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage