Anti-ASIC4 Antibody | Alomone Labs ASC-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASIC4 Antibody

catalog :

ASC-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse ND7/23 neuroblastoma x rat DRG neuron hybrid cell line (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates: - 1,2. Anti-ASIC4 Antibody (#ASC-015), (1:200).3,4. Anti-ASIC4 Antibody, preincubated with ASIC4 Blocking Peptide (#BLP-SC015).

image 2 :

Western blot analysisof ND7/23 cell line (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates: - 12.Anti-ASIC4Antibody (#ASC-015) (1:200).34. Anti-ASIC4 Antibody preincubated with the negative control antigen.

image 3 :

Peptide CKIKFAEEDAKPKEKEAGDE corresponding to amino acid residues 7-26 of rat ASIC4 (AccessionQ9QYV9).Intracellular N-terminus.

product information

CAT :

ASC-015

SKU :

ASC-015-CF_0.2 ml

Product Name :

Anti-ASIC4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QYV9

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC015

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CKIKFAEEDAKPKEKEAGDE, corresponding to amino acid residues 7-26 of rat ASIC4

Product Page - Scientific background :

Acid-sensing ion channels (ASICs) are Na+ channels activated by external protons. ASIC4 is a member of the ASIC family that includes four additional members: ASIC1, ASIC2, ASIC3 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.The functional ASIC channel is composed of 4 subunits, which can form either a homo- or heterotetramer. The subunit composition of the tetrameric channel will determine its biophysical properties such as pH sensitivity, inactivation kinetics and channel pharmacology. In fact, the ASIC4 protein does not appear to function as a proton-gated channel when expressed alone in heterologous systems and therefore a modulatory role for this subunit has been proposed.Expression of the ASIC4 protein has been detected in the brain, pituitary gland, spinal cord and inner ear. The functional significance of ASIC4 expression in these tissues still awaits clarification.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4

Short Description :

A Rabbit Polyclonal Antibody to ASIC4 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat ASIC4. Anti-ASIC4 Antibody (#ASC-015) can be used in western blot analysis. It has been designed to recognize ASIC4 from rat, human and mouse samples.

Negative Control :

BLP-SC015

Positive Control :

Synonyms :

Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage