Anti-ASIC1 Antibody | Alomone Labs ASC-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASIC1 Antibody

catalog :

ASC-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; fig 2c western blot; rat; 1:200; fig 2a	Hu Y, Mi X, Huang C, Wang H, Song J, Shu Q, et al. Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes. Sci Rep. 2017;7:44951 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 6	Ferenczi E, Vierock J, Atsuta Tsunoda K, Tsunoda S, Ramakrishnan C, Gorini C, et al. Optogenetic approaches addressing extracellular modulation of neural excitability. Sci Rep. 2016;6:23947 pubmed publisher
	Li Y, Tian Y, Wu Y, Xu Y, Zhang P, Sha J, et al. Upregulation of Spinal ASIC1 and NKCC1 Expression Contributes to Chronic Visceral Pain in Rats. Front Mol Neurosci. 2020;13:611179 pubmed publisher
	Liu X, Liu C, Ye J, Zhang S, Wang K, Su R. Distribution of Acid Sensing Ion Channels in Axonal Growth Cones and Presynaptic Membrane of Cultured Hippocampal Neurons. Front Cell Neurosci. 2020;14:205 pubmed publisher
	Nomura K, Hiyama T, Sakuta H, Matsuda T, Lin C, Kobayashi K, et al. [Na+] Increases in Body Fluids Sensed by Central Nax Induce Sympathetically Mediated Blood Pressure Elevations via H+-Dependent Activation of ASIC1a. Neuron. 2018;: pubmed publisher
	Stephan G, Huang L, Tang Y, Vilotti S, Fabbretti E, Yu Y, et al. The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nat Commun. 2018;9:1354 pubmed publisher
	Trac P, Thai T, Linck V, Zou L, Greenlee M, Yue Q, et al. Alveolar nonselective channels are ASIC1a/?-ENaC channels and contribute to AFC. Am J Physiol Lung Cell Mol Physiol. 2017;312:L797-L811 pubmed publisher
	Song N, Guan R, Jiang Q, Hassanzadeh C, Chu Y, Zhao X, et al. Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception. Sci Rep. 2016;6:38777 pubmed publisher
	Wu Y, Qin D, Yang H, Fu H. Evidence for the Participation of Acid-Sensing Ion Channels (ASICs) in the Antinociceptive Effect of Curcumin in a Formalin-Induced Orofacial Inflammatory Model. Cell Mol Neurobiol. 2017;37:635-642 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-ASIC1 Antibody (#ASC-014), (1:200). 2. Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).

image 2 :

Expression of ASIC1 in rat brain - Immunohistochemical staining of rat globus pallidus using Anti-ASIC1 Antibody (#ASC-014). A. Parvalbumin (PV) positive neurons are shown (green). B. Neurons with typically enmeshed dendritic trees stain intensely for ASIC1 (red). C. Double labeling with mouse anti-Parvalbumin (green) reveals strong co-localization of PV with ASIC1.

image 3 :

Western blot analysisof rat brain membranes: - 1.Anti-ASIC1 Antibody (#ASC-014) (1:200). 2. Anti-ASIC1 Antibody preincubated with the control peptide antigen.

product information

CAT :

ASC-014

SKU :

ASC-014-CF_0.2 ml

Product Name :

Anti-ASIC1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P55926

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC014

Homology :

Human - identical; mouse - 19/20 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1

Product Page - Scientific background :

ASIC1 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC2, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions. ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium. Recently, ASIC1 has been implicated in cognitive processes such as learning and memory.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2

Short Description :

A Rabbit Polyclonal Antibody to ASIC1 Channel

Long Description :

Anti-ASIC1 Antibody (#ASC-014) is an antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC1 from rat, human, and mouse samples, and recognizes both ASIC1 isoforms.

Negative Control :

BLP-SC014

Positive Control :

Synonyms :

Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC1

AB Specifictiy :

Recognizes isoforms ASIC1α and ASIC1β.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage