Guinea pig Anti-ASIC1 Antibody | Alomone Labs ASC-014-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-ASIC1 Antibody

catalog :

ASC-014-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Zhao Y, Chen Y, Wang Z, Peng C, Li Q, Wu J, et al. The method of Yiqi Yangyin Tongluo can attenuate the pyroptosis of rheumatoid arthritis chondrocytes through the ASIC1a/NLRP3 signaling pathway. Ann Transl Med. 2022;10:145 pubmed publisher
Liu X, Liu C, Ye J, Zhang S, Wang K, Su R. Distribution of Acid Sensing Ion Channels in Axonal Growth Cones and Presynaptic Membrane of Cultured Hippocampal Neurons. Front Cell Neurosci. 2020;14:205 pubmed publisher
Zhang X, Saric T, Mehrjardi N, Hamad S, Morad M. Acid Sensitive Ion channels are expressed in human induced pluripotent stem cell-derived cardiomyocytes. Stem Cells Dev. 2019;: pubmed publisher
Dai B, Zhu F, Chen Y, Zhou R, Wang Z, Xie Y, et al. ASIC1a Promotes Acid-Induced Autophagy in Rat Articular Chondrocytes through the AMPK/FoxO3a Pathway. Int J Mol Sci. 2017;18: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).3,4. Guinea pig Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).

image 2 :

Multiplex staining of ASIC1 and parvalbumin in rat hippocampus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:300). A. ASIC1 (green) is detected in the hilus of the dentate gyrus (arrows). B. The same section was stained with anti-parvalbumin (red). C. Merge of the two images shows partial colocalization (orange, see arrows). Cell nuclei in the same section were stained with DAPI (Blue).

product information

CAT :

ASC-014-GP

SKU :

ASC-014-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-ASIC1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P55926

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-SC014

Homology :

Human - identical; mouse - 19/20 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1

Product Page - Scientific background :

ASIC1 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC2, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions. ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium. Recently, ASIC1 has been implicated in cognitive processes such as learning and memory.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2

Short Description :

A Guinea Pig Polyclonal Antibody to ASIC1 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat ASIC1. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP, formerly AGP-053) raised in guinea pig can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ASIC1 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-ASIC1 Antibody (#ASC-014) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-SC014

Positive Control :

Synonyms :

Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC1

AB Specifictiy :

This antibody will recognize all known ASIC1 isoforms.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage