Anti-NaV1.5 (SCN5A) (1978-2016) Antibody | Alomone Labs ASC-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.5 (SCN5A) (1978-2016) Antibody

catalog :

ASC-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Khoo K, Galleano I, Gasparri F, Wieneke R, Harms H, Poulsen M, et al. Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. Nat Commun. 2020;11:2284 pubmed publisher
Cortada E, Brugada R, Verges M. N-Glycosylation of the voltage-gated sodium channel β2 subunit is required for efficient trafficking of NaV1.5/β2 to the plasma membrane. J Biol Chem. 2019;294:16123-16140 pubmed publisher
Nof E, Vysochek L, Meisel E, Burashnikov E, Antzelevitch C, Clatot J, et al. Mutations in NaV1.5 Reveal Calcium-Calmodulin Regulation of Sodium Channel. Front Physiol. 2019;10:700 pubmed publisher
Yu G, Liu Y, Qin J, Wang Z, Hu Y, Wang F, et al. Mechanistic insights into the interaction of the MOG1 protein with the cardiac sodium channel Nav1.5 clarify the molecular basis of Brugada syndrome. J Biol Chem. 2018;293:18207-18217 pubmed publisher
Wang J, Ou S, Zhang Z, Qiu B, Wang Y. Molecular expression of multiple Nav1.5 splice variants in the frontal lobe of the human brain. Int J Mol Med. 2017;: pubmed publisher
Huang Y, Wang Z, Liu Y, Xiong H, Zhao Y, Wu L, et al. Î±B-Crystallin Interacts with Nav1.5 and Regulates Ubiquitination and Internalization of Cell Surface Nav1.5. J Biol Chem. 2016;291:11030-41 pubmed publisher

image

image 1 :

Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells: - 1. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013), (1:200).2. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody, preincubated with Nav1.5/SCN5A (1978-2016) Blocking Peptide (#BLP-SC013).

image 2 :

Expression of NaV1.5 in rat cardiac muscle - Immunohistochemical staining of NaV1.5 in rat myocardium paraffin-embedded section using Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.

image 3 :

Western blot analysisof NaV1.5 in NaV1.5 transfected HEK-293 cells: - 1.Anti-NaV1.5 (SCN5A) (1978-2016) Antibody(#ASC-013) (1:200).2. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody preincubated with the control peptide antigen.

product information

CAT :

ASC-013

SKU :

ASC-013-CF_0.2 ml

Product Name :

Anti-NaV1.5 (SCN5A) (1978-2016) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q14524

Applications :

IC IFC IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC013

Homology :

Rat - 36/39 amino acid residues identical; mouse - 34/39 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

GST fusion protein with amino acid residues 1978-2016 of human NaV1.5

Product Page - Scientific background :

Voltage-gated Na+ channels (NaV) are responsible for myocardial conduction and maintenance of the cardiac rhythm and are essential for the generation of action potentials and cell excitability.1Dysfunction or disregulation of cardiac sodium channels can cause several disorders, including cardiac arrhythmias. The majority of Na+ channels in the mammalian heart are Tetrodotoxin (TTX) insensitive NaV1.5.2 The putative structure of NaV1.5 consists of four homologous domain (I-IV), each containing six transmembrane segments (S1-S6). Mutations in the C-terminus of NaV1.5 were described in connection to Long QT syndrome and Brugada syndrome.1-2 Recent data have demonstrated selective expression of NaV1.5 in the mouse central nervous system and implicated a role for NaV1.5 in the physiology of the central nervous system.1

Applications may also work in :

IC IFC IHC WB

Supplier :

Alomone Labs

Target :

Sodium channel protein type 5 subunit alpha, SKM2

Short Description :

A Rabbit Polyclonal Antibody to NaV1.5 (SCN5A) (1978-2016) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human NaV1.5 channel. Anti-NaV1.5 (SCN5A) (1978-2016) Antibody (#ASC-013) can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.5 sodium channel from rat, human, and mouse samples.

Negative Control :

BLP-SC013

Positive Control :

Synonyms :

Sodium channel protein type 5 subunit alpha, SKM2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN5A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage