Anti-ASIC2a Antibody | Alomone Labs ASC-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASIC2a Antibody

catalog :

ASC-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; rat; fig 2c western blot; rat; 1:200; fig 2a	Hu Y, Mi X, Huang C, Wang H, Song J, Shu Q, et al. Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes. Sci Rep. 2017;7:44951 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 6	Ferenczi E, Vierock J, Atsuta Tsunoda K, Tsunoda S, Ramakrishnan C, Gorini C, et al. Optogenetic approaches addressing extracellular modulation of neural excitability. Sci Rep. 2016;6:23947 pubmed publisher
	Xu X, Li Y, Wu Y, Xu Y, Weng R, Wang C, et al. Upregulation of spinal ASIC1 by miR-485 mediates enterodynia in adult offspring rats with prenatal maternal stress. CNS Neurosci Ther. 2021;27:244-255 pubmed publisher
	Yang J, Qiu L, Strobel M, Kabel A, Zha X, Chen X. Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid Sensing of Mouse Olfactory Sensory Neurons. Mol Neurobiol. 2020;57:3042-3056 pubmed publisher
	Stephan G, Huang L, Tang Y, Vilotti S, Fabbretti E, Yu Y, et al. The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nat Commun. 2018;9:1354 pubmed publisher
	Song N, Guan R, Jiang Q, Hassanzadeh C, Chu Y, Zhao X, et al. Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception. Sci Rep. 2016;6:38777 pubmed publisher
	Wu Y, Qin D, Yang H, Fu H. Evidence for the Participation of Acid-Sensing Ion Channels (ASICs) in the Antinociceptive Effect of Curcumin in a Formalin-Induced Orofacial Inflammatory Model. Cell Mol Neurobiol. 2017;37:635-642 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-ASIC2a Antibody (#ASC-012), (1:200).2. Anti-ASIC2a Antibody, preincubated with ASIC2a Blocking Peptide (#BLP-SC012).

image 2 :

Expression of ASIC2a in rat spinal cord. - Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-ASIC2a Antibody (#ASC-012), (1:300), followed by donkey anti-rabbit-Cy3. A. ASIC2a immunoreactivity (red) appeared in neuronal soma (horizontal arrow) in the ventral horn of the spinal cord. B. Pre-incubation of the antibody with ASIC2a Blocking Peptide (BLP-SC012), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ASC-012

SKU :

ASC-012-CF_0.2 ml

Product Name :

Anti-ASIC2a Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q16515

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC012

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a

Product Page - Scientific background :

ASIC2a is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.There are two recognized splice variants of the ASIC2 gene that differ on their N-termini, ASIC2a and ASIC2b that have different tissue distributions and functions. ASIC2a is highly expressed in the central nervous system (CNS) and is less expressed in the peripheral nervous system (PNS) while ASIC2b is prominently expressed in the latter.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits. The ASIC2b splice variant does not appear to be functional when expressed alone but it can modify the properties of ASIC2a and ASIC3 when co-expressed.The ASIC2 protein has been proposed to be involved in mechanosensation and sensory transduction.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG

Short Description :

A Rabbit Polyclonal Antibody to ASIC2a Channel

Long Description :

Anti-ASIC2a Antibody (#ASC-012) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ASIC2a from human, rat, and mouse samples. The antibody is specific for ASIC2a and will not recognize the ASIC2b variant.

Negative Control :

BLP-SC012

Positive Control :

Synonyms :

ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC2

AB Specifictiy :

The antibody is specific for ASIC2a and will not recognize ASIC2b.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage