Anti-ASIC2a-ATTO Fluor-594 Antibody | Alomone Labs ASC-012-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASIC2a-ATTO Fluor-594 Antibody

catalog :

ASC-012-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Xu X, Li Y, Wu Y, Xu Y, Weng R, Wang C, et al. Upregulation of spinal ASIC1 by miR-485 mediates enterodynia in adult offspring rats with prenatal maternal stress. CNS Neurosci Ther. 2021;27:244-255 pubmed publisher

image

image 1 :

Expression of ASIC2a in rat cerebellum and spinal cord - Immunohistochemical staining of perfusion-fixed frozen rat brain and spinal cord sections using Anti-ASIC2a-ATTO Fluor-594 Antibody (#ASC-012-AR), (1:60). A. Staining in rat cerebellum sections reveals ASIC2a expression (red) in the molecular layer (MOL) of the cerebellar cortex. B. Staining in rat spinal cord sections shows labeling (red) of neuronal soma (horizontal arrow) in the ventral horn of the spinal cord. DAPI (blue) is used as counterstain in A and B.

image 2 :

Western blot analysis of Rat DRG lysate: - 1. Guinea pigAnti-TRPV1 (VR1)Antibody (#AGP-118) (1:200).2. Guinea pig Anti-TRPV1 (VR1) Antibody preincubated with the negative control antigen.Following a broad screen of secondary antibodies the following were used for these applications: Western blot analysis: #106-035-006 (Jackson ImmunoResearch).

product information

CAT :

ASC-012-AR

SKU :

ASC-012-AR_50 mcl

Product Name :

Anti-ASIC2a-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q16515

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC012

Homology :

Rat, mouse- identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, N-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a

Product Page - Scientific background :

ASIC2a is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.There are two recognized splice variants of the ASIC2 gene that differ on their N-termini, ASIC2a and ASIC2b that have different tissue distributions and functions. ASIC2a is highly expressed in the central nervous system (CNS) and is less expressed in the peripheral nervous system (PNS) while ASIC2b is prominently expressed in the latter.The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits. The ASIC2b splice variant does not appear to be functional when expressed alone but it can modify the properties of ASIC2a and ASIC3 when co-expressed.The ASIC2 protein has been proposed to be involved in mechanosensation and sensory transduction.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG

Short Description :

A Rabbit Polyclonal Antibody to ASIC2a Channel Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-ASIC2a Antibody (#ASC-012) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ASIC2a from human, rat, and mouse samples. The antibody is specific for ASIC2a and will not recognize the ASIC2b variant. Anti-ASIC2a-ATTO Fluor-594 Antibody (#ASC-012-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-ASIC2a-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-SC012

Positive Control :

Synonyms :

ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

ASIC2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ASC-012), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage