Anti-ENaC γ (SCNN1G) (extracellular) Antibody | Alomone Labs ASC-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ENaC γ (SCNN1G) (extracellular) Antibody

catalog :

ASC-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Corrêa J, Boaro K, Sene L, Polidoro J, Salles T, Martins F, et al. Antiproteinuric and Hyperkalemic Mechanisms Activated by Dual Versus Single Blockade of the RAS in Renovascular Hypertensive Rats. Front Physiol. 2021;12:656460 pubmed publisher

image

image 1 :

Western blot analysis of rat lung lysate: - 1. Anti-ENaC γ (SCNN1G) (extracellular) Antibody (#ASC-011), (1:200).2. Anti-ENaC γ (SCNN1G) (extracellular) Antibody, preincubated with ENaC gamma/SCNN1G (extracellular) Blocking Peptide (#BLP-SC011).

image 2 :

Western blot analysisof rat lung lysate: - 1.Anti-ENaC ? (SCNN1G) (extracellular) Antibody(#ASC-011)(1:200).2. Anti-ENaC ? (SCNN1G) (extracellular) Antibody preincubated with the control peptide antigen.

image 3 :

Peptide (C)EDAEVFKDSMVPGEK corresponding to amino acid residues 824-838 of rat TRPV1 (AccessionO35433). Intracellular C-terminus.

product information

CAT :

ASC-011

SKU :

ASC-011-CF_0.2 ml

Product Name :

Anti-ENaC γ (SCNN1G) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P37091

Applications :

IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC011

Homology :

Human - 13/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaCγ

Product Page - Scientific background :

The amiloride-sensitive epithelial Na+ channel (ENaCs) family includes 4 members: ENaCα, β, γ and δ. The ENaC channel is located in the luminal (apical) plasma membrane of several epithelial tissues such as kidney, lung, salivary glands and skin. The functional channel is believed to be a multimer including a α (or δ) and a β and γ subunits with a likely stoichiometry of α2βγ.ENaC channel enable entry of Na+ into the cell along its electrochemical gradient and thus has a central role in the maintenance of renal Na+ balance (and hence blood pressure) and liquid balance in the lung. Indeed, genetic mutations in the ENaC subunits causes Liddle's syndrome (a form of hypertension) or pseudohypoaldosteronism type 1 (PHA) that is characterized by hypotension. The ENaC channel is voltage-independent and is constitutively active in epithelia although it is modulated by several different mechanisms. One of the main mechanisms is the controlled internalization of the channel that is dependent on the β or γ subunits. Indeed, mutations in the C-termini of these subunits reduce endocytosis of the channel leading to the accumulation of ENaC in the cell membrane and causing a phenotype that is consistent with that of Liddle's syndrome.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG

Short Description :

A Rabbit Polyclonal Antibody to ENaC? Channel

Long Description :

Anti-ENaC γ (SCNN1G) Antibody (#ASC-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular domain and can potentially detect the channel in living cells. It has been designed to recognize ENaCγ from rat and human samples.

Negative Control :

BLP-SC011

Positive Control :

Synonyms :

Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCNN1G

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage