product summary
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company name :
Alomone Labs
product type :
antibody
product name :
Anti-NaV1.6 (SCN8A) Antibody
catalog :
ASC-009
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
NA
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
citations: 64
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 6c
Meschkat M, Steyer A, Weil M, Kusch K, Jahn O, Piepkorn L, et al. White matter integrity in mice requires continuous myelin synthesis at the inner tongue. Nat Commun. 2022;13:1163 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5d
Hay C, Jackson S, Mitew S, Scott D, Koenning M, Bensen A, et al. The oligodendrocyte-enriched orphan G protein-coupled receptor Gpr62 is dispensable for central nervous system myelination. Neural Dev. 2021;16:6 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:300; loading ...; fig 5e
Zhang Q, Zhu W, Xu F, Dai X, Shi L, Cai W, et al. The interleukin-4/PPARγ signaling axis promotes oligodendrocyte differentiation and remyelination after brain injury. PLoS Biol. 2019;17:e3000330 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig f2s3b
Erwig M, Patzig J, Steyer A, Dibaj P, Heilmann M, Heilmann I, et al. Anillin facilitates septin assembly to prevent pathological outfoldings of central nervous system myelin. elife. 2019;8: pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1n
Goebbels S, Wieser G, Pieper A, Spitzer S, Weege B, Yan K, et al. A neuronal PI(3,4,5)P3-dependent program of oligodendrocyte precursor recruitment and myelination. Nat Neurosci. 2017;20:10-15 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:300; fig 8
Alshammari M, Alshammari T, Laezza F. Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment. Front Cell Neurosci. 2016;10:5 pubmed publisher
Jo Y, Lee J, Lee S, Kwon I, Cho H. Poly-dipeptides produced from C9orf72 hexanucleotide repeats cause selective motor neuron hyperexcitability in ALS. Proc Natl Acad Sci U S A. 2022;119:e2113813119 pubmed publisher
Mulpuri Y, Yamamoto T, Nishimura I, Spigelman I. Role of voltage-gated sodium channels in axonal signal propagation of trigeminal ganglion neurons after infraorbital nerve entrapment. Neurobiol Pain. 2022;11:100084 pubmed publisher
Albrecht P, Houk G, Ruggiero E, Dockum M, Czerwinski M, Betts J, et al. Keratinocyte Biomarkers Distinguish Painful Diabetic Peripheral Neuropathy Patients and Correlate With Topical Lidocaine Responsiveness. Front Pain Res (Lausanne). 2021;2:790524 pubmed publisher
Piccialli I, Ciccone R, Secondo A, Boscia F, Tedeschi V, de Rosa V, et al. The Na+/Ca2+ Exchanger 3 Is Functionally Coupled With the NaV1.6 Voltage-Gated Channel and Promotes an Endoplasmic Reticulum Ca2+ Refilling in a Transgenic Model of Alzheimer's Disease. Front Pharmacol. 2021;12:775271 pubmed publisher
Lillo M, Gaete P, Puebla M, Burboa P, Poblete I, Figueroa X. Novel Pannexin-1-Coupled Signaling Cascade Involved in the Control of Endothelial Cell Function and NO-Dependent Relaxation. Oxid Med Cell Longev. 2021;2021:2678134 pubmed publisher
Hu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, et al. Flotillin-1 Interacts With and Sustains the Surface Levels of TRPV2 Channel. Front Cell Dev Biol. 2021;9:634160 pubmed publisher
Alam M, Zhao X, Liao Y, Mathur R, McCallum S, Mazurkiewicz J, et al. Deficiency of Microglial Autophagy Increases the Density of Oligodendrocytes and Susceptibility to Severe Forms of Seizures. Eneuro. 2021;8: pubmed publisher
Karahuseyinoglu S, Sekerdag E, Aria M, Cetin Tas Y, Nizamoglu S, Solaroglu I, et al. Three-dimensional neuron-astrocyte construction on matrigel enhances establishment of functional voltage-gated sodium channels. J Neurochem. 2021;156:848-866 pubmed publisher
Housley S, Nardelli P, Carrasco D, Rotterman T, Pfahl E, Matyunina L, et al. Cancer Exacerbates Chemotherapy-Induced Sensory Neuropathy. Cancer Res. 2020;80:2940-2955 pubmed publisher
Mi Z, Yang J, He Q, Zhang X, Xiao Y, Shu Y. Alterations of Electrophysiological Properties and Ion Channel Expression in Prefrontal Cortex of a Mouse Model of Schizophrenia. Front Cell Neurosci. 2019;13:554 pubmed publisher
Plantier V, Sanchez Brualla I, Dingu N, Brocard C, Liabeuf S, Gackiere F, et al. Calpain fosters the hyperexcitability of motoneurons after spinal cord injury and leads to spasticity. elife. 2019;8: pubmed publisher
Ciccone R, Franco C, Piccialli I, Boscia F, Casamassa A, de Rosa V, et al. Amyloid β-Induced Upregulation of Nav1.6 Underlies Neuronal Hyperactivity in Tg2576 Alzheimer's Disease Mouse Model. Sci Rep. 2019;9:13592 pubmed publisher
Seki S, Yamamoto T, Quinn K, Spigelman I, Pantazis A, Olcese R, et al. Circuit-Specific Early Impairment of Proprioceptive Sensory Neurons in the SOD1G93A Mouse Model for ALS. J Neurosci. 2019;39:8798-8815 pubmed publisher
Vysokov N, McMahon S, Raouf R. The role of NaV channels in synaptic transmission after axotomy in a microfluidic culture platform. Sci Rep. 2019;9:12915 pubmed publisher
Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
Zhou Y, Xia C, Yin M, Wang X, Wu H, Ji Y. Distribution and Functional Characteristics of Voltage-Gated Sodium Channels in Immature Cochlear Hair Cells. Neurosci Bull. 2020;36:49-65 pubmed publisher
Fröb F, Sock E, Tamm E, Saur A, Hillgärtner S, Williams T, et al. Ep400 deficiency in Schwann cells causes persistent expression of early developmental regulators and peripheral neuropathy. Nat Commun. 2019;10:2361 pubmed publisher
Tai Y, Gallo N, Wang M, Yu J, Van Aelst L. Axo-axonic Innervation of Neocortical Pyramidal Neurons by GABAergic Chandelier Cells Requires AnkyrinG-Associated L1CAM. Neuron. 2019;102:358-372.e9 pubmed publisher
Ding H, Zhang S, Lv Y, Ma C, Liu M, Zhang K, et al. TNF-α/STAT3 pathway epigenetically upregulates Nav1.6 expression in DRG and contributes to neuropathic pain induced by L5-VRT. J Neuroinflammation. 2019;16:29 pubmed publisher
Ding Y, Chen T, Wang Q, Yuan Y, Hua T. Axon initial segment plasticity accompanies enhanced excitation of visual cortical neurons in aged rats. Neuroreport. 2018;29:1537-1543 pubmed publisher
Lopez Charcas O, Espinosa A, Alfaro A, Herrera Carrillo Z, Ramírez Cordero B, Cortes Reynosa P, et al. The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity. Sci Rep. 2018;8:12995 pubmed publisher
Marion C, Radomski K, Cramer N, Galdzicki Z, Armstrong R. Experimental Traumatic Brain Injury Identifies Distinct Early and Late Phase Axonal Conduction Deficits of White Matter Pathophysiology, and Reveals Intervening Recovery. J Neurosci. 2018;38:8723-8736 pubmed publisher
Wang F, Yang Y, Yang N, Chen X, Huang N, Zhang J, et al. Enhancing Oligodendrocyte Myelination Rescues Synaptic Loss and Improves Functional Recovery after Chronic Hypoxia. Neuron. 2018;99:689-701.e5 pubmed publisher
Kaczmarek Hájek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, et al. Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody. elife. 2018;7: pubmed publisher
Xie A, Gallant B, Guo H, Gonzalez A, Clark M, Madigan A, et al. Functional cardiac Na+ channels are expressed in human melanoma cells. Oncol Lett. 2018;16:1689-1695 pubmed publisher
Katz E, Stoler O, Scheller A, Khrapunsky Y, Goebbels S, Kirchhoff F, et al. Role of sodium channel subtype in action potential generation by neocortical pyramidal neurons. Proc Natl Acad Sci U S A. 2018;115:E7184-E7192 pubmed publisher
Koleske M, Bonilla I, Thomas J, Zaman N, Baine S, Knollmann B, et al. Tetrodotoxin-sensitive Navs contribute to early and delayed afterdepolarizations in long QT arrhythmia models. J Gen Physiol. 2018;150:991-1002 pubmed publisher
Luo F, Zhang J, Burke K, Romito Digiacomo R, Miller R, Yang Y. Oligodendrocyte-specific loss of Cdk5 disrupts the architecture of nodes of Ranvier as well as learning and memory. Exp Neurol. 2018;306:92-104 pubmed publisher
Ali S, Liu Z, Nenov M, Folorunso O, Singh A, Scala F, et al. Functional Modulation of Voltage-Gated Sodium Channels by a FGF14-Based Peptidomimetic. ACS Chem Neurosci. 2018;9:976-987 pubmed publisher
Wang T, van Woerden G, Elgersma Y, Borst J. Enhanced Transmission at the Calyx of Held Synapse in a Mouse Model for Angelman Syndrome. Front Cell Neurosci. 2017;11:418 pubmed publisher
Bang M, Vainshtein A, Yang H, Eshed Eisenbach Y, Devaux J, Werner H, et al. Glial M6B stabilizes the axonal membrane at peripheral nodes of Ranvier. Glia. 2018;66:801-812 pubmed publisher
Michelson N, Vazquez A, Eles J, Salatino J, Purcell E, Williams J, et al. Multi-scale, multi-modal analysis uncovers complex relationship at the brain tissue-implant neural interface: New Emphasis on the Biological Interface. J Neural Eng. 2017;: pubmed publisher
Browne L, Smith K, Jagger D. Identification of Persistent and Resurgent Sodium Currents in Spiral Ganglion Neurons Cultured from the Mouse Cochlea. Eneuro. 2017;4: pubmed publisher
Hu L, Wang B, Zhang Y. Serotonin 5-HT6 receptors affect cognition in a mouse model of Alzheimer's disease by regulating cilia function. Alzheimers Res Ther. 2017;9:76 pubmed publisher
Berret E, Barron T, Xu J, Debner E, Kim E, Kim J. Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation. Nat Commun. 2017;8:557 pubmed publisher
Barker B, Nigam A, Ottolini M, Gaykema R, Hargus N, Patel M. Pro-excitatory alterations in sodium channel activity facilitate subiculum neuron hyperexcitability in temporal lobe epilepsy. Neurobiol Dis. 2017;108:183-194 pubmed publisher
Liu Y, Lai S, Ma W, Ke W, Zhang C, Liu S, et al. CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression. Nat Commun. 2017;8:355 pubmed publisher
Johnson K, Herold K, Milner T, Hemmings H, Platholi J. Sodium channel subtypes are differentially localized to pre- and post-synaptic sites in rat hippocampus. J Comp Neurol. 2017;525:3563-3578 pubmed publisher
Ottolini M, Barker B, Gaykema R, Meisler M, Patel M. Aberrant Sodium Channel Currents and Hyperexcitability of Medial Entorhinal Cortex Neurons in a Mouse Model of SCN8A Encephalopathy. J Neurosci. 2017;37:7643-7655 pubmed publisher
Davidson N, Yu F, Kijpaisalratana N, Le T, Beer L, Radomski K, et al. Leukemia/lymphoma-related factor (LRF) exhibits stage- and context-dependent transcriptional controls in the oligodendrocyte lineage and modulates remyelination. J Neurosci Res. 2017;95:2391-2408 pubmed publisher
Anderson L, Hawkins N, Thompson C, Kearney J, George A. Unexpected Efficacy of a Novel Sodium Channel Modulator in Dravet Syndrome. Sci Rep. 2017;7:1682 pubmed publisher
Kleinecke S, Richert S, de Hoz L, Brügger B, Kungl T, Asadollahi E, et al. Peroxisomal dysfunctions cause lysosomal storage and axonal Kv1 channel redistribution in peripheral neuropathy. elife. 2017;6: pubmed publisher
Bolz F, Kasper S, Bufe B, Zufall F, Pyrski M. Organization and Plasticity of Sodium Channel Expression in the Mouse Olfactory and Vomeronasal Epithelia. Front Neuroanat. 2017;11:28 pubmed publisher
Sierksma M, Borst J. Resistance to action potential depression of a rat axon terminal in vivo. Proc Natl Acad Sci U S A. 2017;114:4249-4254 pubmed publisher
Hamm V, H xe9 raud C, Bott J, Herbeaux K, Strittmatter C, Mathis C, et al. Differential contribution of APP metabolites to early cognitive deficits in a TgCRND8 mouse model of Alzheimer's disease. Sci Adv. 2017;3:e1601068 pubmed publisher
Makinson C, Tanaka B, Sorokin J, Wong J, Christian C, Goldin A, et al. Regulation of Thalamic and Cortical Network Synchrony by Scn8a. Neuron. 2017;93:1165-1179.e6 pubmed publisher
Amor V, Zhang C, Vainshtein A, Zhang A, Zollinger D, Eshed Eisenbach Y, et al. The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier. elife. 2017;6: pubmed publisher
Arancibia Carcamo I, Ford M, Cossell L, Ishida K, Tohyama K, Attwell D. Node of Ranvier length as a potential regulator of myelinated axon conduction speed. elife. 2017;6: pubmed publisher
Carrasco D, Vincent J, Cope T. Distribution of TTX-sensitive voltage-gated sodium channels in primary sensory endings of mammalian muscle spindles. J Neurophysiol. 2017;117:1690-1701 pubmed publisher
Valkova C, Liebmann L, Kramer A, Hübner C, Kaether C. The sorting receptor Rer1 controls Purkinje cell function via voltage gated sodium channels. Sci Rep. 2017;7:41248 pubmed publisher
Zhu H, Zhao Y, Wu H, Jiang N, Wang Z, Lin W, et al. Remarkable alterations of Nav1.6 in reactive astrogliosis during epileptogenesis. Sci Rep. 2016;6:38108 pubmed publisher
Stahon K, Bastian C, Griffith S, Kidd G, Brunet S, Baltan S. Age-Related Changes in Axonal and Mitochondrial Ultrastructure and Function in White Matter. J Neurosci. 2016;36:9990-10001 pubmed publisher
Yang K, Kim M, Ju J, Park S, Lee C, Kim S, et al. Antinociceptive Effects of Botulinum Toxin Type A on Trigeminal Neuropathic Pain. J Dent Res. 2016;95:1183-90 pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher
Brocard C, Plantier V, Boulenguez P, Liabeuf S, Bouhadfane M, Viallat Lieutaud A, et al. Cleavage of Na(+) channels by calpain increases persistent Na(+) current and promotes spasticity after spinal cord injury. Nat Med. 2016;22:404-11 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, Schnöbel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher
Yin L, Rasch M, He Q, Wu S, Dou F, Shu Y. Selective Modulation of Axonal Sodium Channel Subtypes by 5-HT1A Receptor in Cortical Pyramidal Neuron. Cereb Cortex. 2017;27:509-521 pubmed publisher
Blanchard M, Willemsen M, Walker J, Dib Hajj S, Waxman S, Jongmans M, et al. De novo gain-of-function and loss-of-function mutations of SCN8A in patients with intellectual disabilities and epilepsy. J Med Genet. 2015;52:330-7 pubmed publisher
image
image 1 :
Alomone Labs ASC-009 image 1
Western blot analysis of rat brain membrane: - 1. Anti-NaV1.6 (SCN8A) Antibody (#ASC-009), (1:200).2. Anti-NaV1.6 (SCN8A) Antibody, preincubated with Nav1.6/SCN8A Blocking Peptide (#BLP-SC009).
image 2 :
Alomone Labs ASC-009 image 2
Expression of NaV1.6 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus using Anti-NaV1.6 (SCN8A) Antibody (#ASC-009). A. NaV1.6 (green) is robustly expressed in the CA1 pyramidal layer (white arrows). B. Staining with mouse anti-parvalbumin (red), a marker of interneurons. C. Merged image of panels A and B reveals that NaV1.6 appears in some interneurons (arrow) but is not restricted to interneurons. DAPI is used as the counterstain.
image 3 :
Alomone Labs ASC-009 image 3
Peptide CIANHTGVDIHRNGDFQKNG corresponding to amino acid residues 1042-1061 of rat NaV1.6 (AccessionO88420). Intracellular loop between domains II and III.
product information
CAT :
ASC-009
SKU :
ASC-009-CF_0.2 ml
Product Name :
Anti-NaV1.6 (SCN8A) Antibody
Group Type :
Antibodies
Product Type :
Antibodies
Clonality :
Polyclonal
Accession :
O88420
Applications :
IC IF IHC WB
Reactivity :
Human Rat Mouse
Host :
Rabbit
Blocking Peptide :
BLP-SC009
Homology :
Mouse - identical; human - 19/20 amino acid residues identical
Formulation :
PBS pH7.4
isotype :
Rabbit IgG
Peptide confirmation :
Confirmed by amino acid analysis and mass spectrometry
Reconstitution :
0.2 ml double distilled water (DDW).
Antibody Concentration After Reconstitut ... :
1 mg/ml
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preservative :
No Preservative
Immunogen Location :
Intracellular loop between domains II and III
Label :
Unconjugated
Storage Before Reconstitution :
The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
immunogen source species :
Rat
Sequence :
CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat NaV1.6
Product Page - Scientific background :
Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. Expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7NaV1.6 is highly expressed in the adult brain and localized at high density in Nodes of Ranvier and axon initial segments and at lower density in dendrites and cell bodies of some neurons. NaV1.6 channels are also expressed at high levels in cerebellar Purkinje neurons.8-11
Applications may also work in :
IC IF IHC WB
Supplier :
Alomone Labs
Target :
Sodium channel voltage-gated type VIII alpha subunit, NaCh6, PN4, CerIII
Short Description :
A Rabbit Polyclonal Antibody to NaV1.6 (SCN8A) Channel
Long Description :
Anti-NaV1.6 (SCN8A) Antibody (#ASC-009) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.6 sodium channel from rat, human, and mouse samples.
Negative Control :
BLP-SC009
Positive Control :
NA
Synonyms :
Sodium channel voltage-gated type VIII alpha subunit, NaCh6, PN4, CerIII
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Specifictiy :
SCN8A
Form :
Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Comment :
Contact Alomone Labs for technical support and product customization
Species reactivity key :
H- Human, M- Mouse, R- Rat
Is Toxin :
No
Purity :
Affinity purified on immobilized antigen.
UNSPSC :
41116161
KO-Validated :
yes
Cited Application :
IP IHC ICC
Clone :
NA
Standard quality control of each lot :
Western blot analysis
Antigen preadsorption control :
1 µg peptide per 1 µg antibody
Application Dilutions: Immunohistochemis ... :
Contact Alomone for information
Application Dilutions: Western blot wb :
1:200
more info or order :
company information
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972 2 531 8002
headquarters: Israel