Anti-NaV1.7 (SCN9A) Antibody | Alomone Labs ASC-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NaV1.7 (SCN9A) Antibody

catalog :

ASC-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 18

Reference
Zeidler M, Kummer K, Schöpf C, Kalpachidou T, Kern G, Cader M, et al. NOCICEPTRA: Gene and microRNA Signatures and Their Trajectories Characterizing Human iPSC-Derived Nociceptor Maturation. Adv Sci (Weinh). 2021;8:e2102354 pubmed publisher
Blom A, van den Bosch M, Blaney Davidson E, Roth J, Vogl T, van de Loo F, et al. The alarmins S100A8 and S100A9 mediate acute pain in experimental synovitis. Arthritis Res Ther. 2020;22:199 pubmed publisher
Paul D, Maggi P, Piero F, Scahill S, Sherman K, Edenfield S, et al. Targeted Osmotic Lysis of Highly Invasive Breast Carcinomas Using Pulsed Magnetic Field Stimulation of Voltage-Gated Sodium Channels and Pharmacological Blockade of Sodium Pumps. Cancers (Basel). 2020;12: pubmed publisher
Housley S, Nardelli P, Carrasco D, Rotterman T, Pfahl E, Matyunina L, et al. Cancer Exacerbates Chemotherapy-Induced Sensory Neuropathy. Cancer Res. 2020;80:2940-2955 pubmed publisher
Zhang Q, Martin Caraballo M, Hsia S. Modulation of Voltage-Gated Sodium Channel Activity in Human Dorsal Root Ganglion Neurons by Herpesvirus Quiescent Infection. J Virol. 2020;94: pubmed publisher
Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
Zhang F, Wang Y, Liu Y, Han H, Zhang D, Fan X, et al. Transcriptional regulation of voltage-gated sodium channels contributes to GM-CSF induced pain. J Neurosci. 2019;: pubmed publisher
Sun J, Li N, Duan G, Liu Y, Guo S, Wang C, et al. Increased Nav1.7 expression in the dorsal root ganglion contributes to pain hypersensitivity after plantar incision in rats. Mol Pain. 2018;14:1744806918782323 pubmed publisher
Das V, Kc R, Li X, Varma D, Qiu S, Kroin J, et al. Pharmacological targeting of the mammalian clock reveals a novel analgesic for osteoarthritis-induced pain. Gene. 2018;655:1-12 pubmed publisher
Warnier M, Flaman J, Chouabe C, Wiel C, Gras B, Griveau A, et al. The SCN9A channel and plasma membrane depolarization promote cellular senescence through Rb pathway. Aging Cell. 2018;17:e12736 pubmed publisher
Li Y, North R, Rhines L, Tatsui C, Rao G, Edwards D, et al. DRG Voltage-Gated Sodium Channel 1.7 Is Upregulated in Paclitaxel-Induced Neuropathy in Rats and in Humans with Neuropathic Pain. J Neurosci. 2018;38:1124-1136 pubmed publisher
Yamane M, Yamashita N, Hida T, Kamiya Y, Nakamura F, Kolattukudy P, et al. A functional coupling between CRMP1 and Nav1.7 for retrograde propagation of Semaphorin3A signaling. J Cell Sci. 2017;130:1393-1403 pubmed publisher
Carrasco D, Vincent J, Cope T. Distribution of TTX-sensitive voltage-gated sodium channels in primary sensory endings of mammalian muscle spindles. J Neurophysiol. 2017;117:1690-1701 pubmed publisher
Yang K, Kim M, Ju J, Park S, Lee C, Kim S, et al. Antinociceptive Effects of Botulinum Toxin Type A on Trigeminal Neuropathic Pain. J Dent Res. 2016;95:1183-90 pubmed publisher
Liu Z, Wang F, Fischer G, Hogan Q, Yu H. Peripheral nerve injury induces loss of nociceptive neuron-specific G?i-interacting protein in neuropathic pain rat. Mol Pain. 2016;12: pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher
Yan J, Zou K, Liu X, Hu S, Wang Q, Miao X, et al. Hyperexcitability and sensitization of sodium channels of dorsal root ganglion neurons in a rat model of lumber disc herniation. Eur Spine J. 2016;25:177-85 pubmed publisher

image

image 1 :

Western blot analysis of DRG lysates: - 1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).2. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).

image 2 :

Expression of NaV1.7 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A) Antibody (#ASC-008 ). A. NaV1.7 channel (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of NaV1.7 and Parvalbumin demonstrates colocalization (arrows).

image 3 :

Peptide (C)EFTSIGRSR IMGLSE corresponding to amino acid residues 446-460 of rat NaV1.7(AccessionO08562). Intracellular loop between domains I and II.

product information

CAT :

ASC-008

SKU :

ASC-008-CF_0.2 ml

Product Name :

Anti-NaV1.7 (SCN9A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08562

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC008

Homology :

Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains I and II

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Short Description :

A Rabbit Polyclonal Antibody to NaV1.7 (SCN9A) Channel

Long Description :

Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.7 from rat, human, and mouse samples.

Negative Control :

BLP-SC008

Positive Control :

Synonyms :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN9A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage