Guinea pig Anti-NaV1.7 (SCN9A) Antibody | Alomone Labs ASC-008-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-NaV1.7 (SCN9A) Antibody

catalog :

ASC-008-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Salehi M, Neumann I, Jurek B, Pandamooz S. Co-Stimulation of Oxytocin and Arginine-Vasopressin Receptors Affect Hypothalamic Neurospheroid Size. Int J Mol Sci. 2021;22: pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysates: - 1. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#ASC-008-GP), (1:400).2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008). Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.

product information

CAT :

ASC-008-GP

SKU :

ASC-008-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-NaV1.7 (SCN9A) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08562

Applications :

Reactivity :

Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-SC008

Homology :

Human, mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains I and II

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Short Description :

A Guinea Pig Polyclonal Antibody to NaV1.7 (SCN9A) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NaV1.7 channel. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#ASC-008-GP, formerly AGP-057) raised in guinea pigs can be used in western blot analysis. It has been designed to recognize NaV1.7 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-SC008

Positive Control :

Synonyms :

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN9A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage